Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1

Yue Guan, Mohamed F. Haroon, Intikhab Alam, James G. Ferry, Ulrich Stingl

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines.

Original languageEnglish (US)
Pages (from-to)404-410
Number of pages7
JournalEnvironmental Microbiology Reports
Volume9
Issue number4
DOIs
StatePublished - Aug 2017

Fingerprint

Mediterranean Sea
Lakes
Genomics
brine
genomics
stop codon
Archaea
lakes
Corrinoids
lake
genome
Terminator Codon
methylamine
methanogenesis
methyltransferases
cells
methane production
amines
multigene family
Archaeal Genome

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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abstract = "Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines.",
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Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1. / Guan, Yue; Haroon, Mohamed F.; Alam, Intikhab; Ferry, James G.; Stingl, Ulrich.

In: Environmental Microbiology Reports, Vol. 9, No. 4, 08.2017, p. 404-410.

Research output: Contribution to journalArticle

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AU - Guan, Yue

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AU - Stingl, Ulrich

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