Single-cell lipidomics: Characterizing and imaging lipids on the surface of individual Aplysia californica neurons with cluster secondary ion mass spectrometry

Melissa K. Passarelli, Andrew G. Ewing, Nicholas Winograd

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Neurons isolated from Aplysia californica, an organism with a well-defined neural network, were imaged with secondary ion mass spectrometry, C 60-SIMS. A major lipid component of the neuronal membrane was identified as 1-hexadecyl-2-octadecenoyl-sn-glycero-3-phosphocholine [PC(16:0e/18:1)] using tandem mass spectrometry (MS/MS). The assignment was made directly off the sample surface using a C60-QSTAR instrument, a prototype instrument that combines an ion source with a commercial electrospray ionization/matrix-assisted laser desorption ionization (ESI/MALDI) mass spectrometer. Normal phase liquid chromatography mass spectrometry (NP-LC-MS) was used to confirm the assignment. Cholesterol and vitamin E were also identified with in situ tandem MS analyses that were compared to reference spectra obtained from purified compounds. In order to improve sensitivity on the single-cell level, the tandem MS spectrum of vitamin E reference material was used to extract and compile all the vitamin E related peaks from the cell image. The mass spectrometry images reveal heterogeneous distributions of intact lipid species, PC(16:0e/18:1), vitamin E, and cholesterol on the surface of a single neuron. The ability to detect these molecules and determine their relative distribution on the single-cell level shows that the C60-QSTAR is a potential platform for studying important biochemical processes, such as neuron degeneration.

Original languageEnglish (US)
Pages (from-to)2231-2238
Number of pages8
JournalAnalytical Chemistry
Volume85
Issue number4
DOIs
StatePublished - Feb 19 2013

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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