Single cell transfection dynamics in a microfluidic system

Na Li, Pak Kin Wong, James Lin, Chih Ming Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In this study, molecular beacons have been successfully transfected into living cells with high efficiency (80%∼90%) inside a microfluidic system. This will enable a sensitive and specific method to monitor gene expression dynamics in single living cells. Fluorescence conjugated transfection reagent was adopted to investigate the transfection dynamics of molecular beacons. The development of microfluidic systems has facilitated real-time monitoring of individual cells, which revealed the transfection dynamics of molecular beacons at the single cell level.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages969-971
Number of pages3
StatePublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Fingerprint

Microfluidics
Cells
Gene expression
Fluorescence
Monitoring

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Li, N., Wong, P. K., Lin, J., & Ho, C. M. (2006). Single cell transfection dynamics in a microfluidic system. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 969-971). Japan Academic Association Inc.
Li, Na ; Wong, Pak Kin ; Lin, James ; Ho, Chih Ming. / Single cell transfection dynamics in a microfluidic system. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 969-971
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Li, N, Wong, PK, Lin, J & Ho, CM 2006, Single cell transfection dynamics in a microfluidic system. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 969-971, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06.

Single cell transfection dynamics in a microfluidic system. / Li, Na; Wong, Pak Kin; Lin, James; Ho, Chih Ming.

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 969-971.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Li N, Wong PK, Lin J, Ho CM. Single cell transfection dynamics in a microfluidic system. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 969-971