Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing

Xiaole Mao, Sz Chin Steven Lin, Cheng Dong, Tony Jun Huang

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

In this work, we demonstrate an on-chip microfluidic flow cytometry system based on a three-dimensional (3D) hydrodynamic focusing technique, microfluidic drifting. By inducing Dean flow in a curved microfluidic channel, microfluidic drifting can be used to hydrodynamically focus cells or particles in the vertical direction and enables the 3D hydrodynamic focusing in a single-layer planar microfluidic device. Through theoretical calculation, numerical simulation, and experimental characterization, we found that the microfluidic drifting technique can be effectively applied to three-dimensionally focus microparticles with density and size equivalent to those of human CD4+ T lymphocytes. In addition, we developed a flow cytometry platform by integrating the 3D focusing device with a laser-induced fluorescence (LIF) detection system. The system was shown to provide effective high-throughput flow cytometry measurements at a rate of greater than 1700 cells s-1.

Original languageEnglish (US)
Pages (from-to)1583-1589
Number of pages7
JournalLab on a Chip
Volume9
Issue number11
DOIs
StatePublished - Jan 1 2009

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Microfluidics
Hydrodynamics
Flow cytometry
Flow Cytometry
Lab-On-A-Chip Devices
T-cells
Lasers
Fluorescence
T-Lymphocytes
Equipment and Supplies
Throughput
Computer simulation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Mao, Xiaole ; Lin, Sz Chin Steven ; Dong, Cheng ; Huang, Tony Jun. / Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. In: Lab on a Chip. 2009 ; Vol. 9, No. 11. pp. 1583-1589.
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Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. / Mao, Xiaole; Lin, Sz Chin Steven; Dong, Cheng; Huang, Tony Jun.

In: Lab on a Chip, Vol. 9, No. 11, 01.01.2009, p. 1583-1589.

Research output: Contribution to journalArticle

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