Single molecule transport characterization of a high permeable artificial water channel

Yue Xiao Shen, Mustafa Erbakan, Creedon W. Meminger, Junli Hou, Manish Kumar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bioinspired artificial channels can combine high stability with the high permeability and selectivity found in biological channels. They have potential applications in next-generation energy-efficient and stable aqueous separation applications. This study is focused on a new architecture of artificial water channels-peptide-appended pillar[5]arenes. This study combined stopped-flow measurement with a newly developed fluorescence correlation spectroscopy technique to, for the first time, successfully determined the single channel water permeability of artificial channels. The peptide-appended pillar[5]arene channel permeability was determined to be (2.81±0.11)×10-17 cm3/s per channel or (9.68±0.38)×105 H2O molecules/s per channel. This permeability value is only 2 magnitudes lower than that of natural water channel-aquaporins, when the low cross-sectional area (0.65 nm2) is compared to larger cross sectional area biological water channels (∼10 nm2). This is an orders of magnitude improvement over the first-generation of the artificial water channels reported before and is the fastest one reported so far. This channel was found to have a pore size of approximately 450 Da and showed ion selectivity in the order of NH4 + < Cs+ < Rb+ < K+ < Na+ < Li+ < Cl- as determined by patch clamp studies. The ability to further chemically modify the versatile chemical architecture of the pillar[5]arene channels shows promise for further improving water permeability and selectivity.

Original languageEnglish (US)
Title of host publicationSeparations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
PublisherAIChE
Pages895-903
Number of pages9
ISBN (Electronic)9781510812727
StatePublished - Jan 1 2014
EventSeparations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting - Atlanta, United States
Duration: Nov 16 2014Nov 21 2014

Publication series

NameSeparations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
Volume2

Other

OtherSeparations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting
CountryUnited States
CityAtlanta
Period11/16/1411/21/14

Fingerprint

Aquaporins
Molecules
Water
Peptides
Clamping devices
Flow measurement
Pore size
Fluorescence
Spectroscopy
Ions

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Energy(all)
  • Chemical Engineering(all)

Cite this

Shen, Y. X., Erbakan, M., Meminger, C. W., Hou, J., & Kumar, M. (2014). Single molecule transport characterization of a high permeable artificial water channel. In Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting (pp. 895-903). (Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; Vol. 2). AIChE.
Shen, Yue Xiao ; Erbakan, Mustafa ; Meminger, Creedon W. ; Hou, Junli ; Kumar, Manish. / Single molecule transport characterization of a high permeable artificial water channel. Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, 2014. pp. 895-903 (Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting).
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abstract = "Bioinspired artificial channels can combine high stability with the high permeability and selectivity found in biological channels. They have potential applications in next-generation energy-efficient and stable aqueous separation applications. This study is focused on a new architecture of artificial water channels-peptide-appended pillar[5]arenes. This study combined stopped-flow measurement with a newly developed fluorescence correlation spectroscopy technique to, for the first time, successfully determined the single channel water permeability of artificial channels. The peptide-appended pillar[5]arene channel permeability was determined to be (2.81±0.11)×10-17 cm3/s per channel or (9.68±0.38)×105 H2O molecules/s per channel. This permeability value is only 2 magnitudes lower than that of natural water channel-aquaporins, when the low cross-sectional area (0.65 nm2) is compared to larger cross sectional area biological water channels (∼10 nm2). This is an orders of magnitude improvement over the first-generation of the artificial water channels reported before and is the fastest one reported so far. This channel was found to have a pore size of approximately 450 Da and showed ion selectivity in the order of NH4 + < Cs+ < Rb+ < K+ < Na+ < Li+ < Cl- as determined by patch clamp studies. The ability to further chemically modify the versatile chemical architecture of the pillar[5]arene channels shows promise for further improving water permeability and selectivity.",
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Shen, YX, Erbakan, M, Meminger, CW, Hou, J & Kumar, M 2014, Single molecule transport characterization of a high permeable artificial water channel. in Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting, vol. 2, AIChE, pp. 895-903, Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting, Atlanta, United States, 11/16/14.

Single molecule transport characterization of a high permeable artificial water channel. / Shen, Yue Xiao; Erbakan, Mustafa; Meminger, Creedon W.; Hou, Junli; Kumar, Manish.

Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, 2014. p. 895-903 (Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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M3 - Conference contribution

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Shen YX, Erbakan M, Meminger CW, Hou J, Kumar M. Single molecule transport characterization of a high permeable artificial water channel. In Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE. 2014. p. 895-903. (Separations Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting).