Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya

Giselle Nick, Philippe De Lajudie, Bertrand D. Eardly, Sini Suomalainen, Lars Paulin, Xiaoping Zhang, Monique Gillis, Kristina Lindstrom

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

SDS-PAGE of total bacterial proteins was applied to the classification of 25 Sudanese and five Kenyan strains isolated from the root nodules of Acacia senegal and Prosopis chilensis. Twenty strains were also studied by multilocus enzyme electrophoresis (MLEE) and the whole 16S rRNA gene was sequenced from two strains representing the two major clusters. These results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, DNA-DNA dot-blot hybridization, genomic fingerprinting using repetitive sequence-based PCR, DNA base composition analysis, DNA-DNA reassociation analysis, partial sequencing of the 16S rRNA gene and RFLP analysis of the amplified 16S rRNA gene, showed that all 30 strains belong to the genus Sinorhizobium. Two of the strains grouped with Sinorhizobium saheli and seven with Sinorhizobium terangae, while the rest did not cluster with any of the established species. The majority of the strains formed two phenotypically and genotypically distinct groups and we therefore propose that these strains should be classified as two new species, Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov.

Original languageEnglish (US)
Pages (from-to)1359-1368
Number of pages10
JournalInternational Journal of Systematic Bacteriology
Volume49
Issue number4
DOIs
StatePublished - Jan 1 1999

Fingerprint

Sinorhizobium kostiense
Sinorhizobium
Sudan
Kenya
DNA
rRNA Genes
new species
gene
electrokinesis
ribosomal RNA
Prosopis
Sinorhizobium saheli
Sinorhizobium terangae
Nucleic Acid Hybridization
Acacia
Senegal
Bacterial Proteins
Nucleic Acid Repetitive Sequences
Pulsed Field Gel Electrophoresis
Prosopis chilensis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology

Cite this

Nick, Giselle ; De Lajudie, Philippe ; Eardly, Bertrand D. ; Suomalainen, Sini ; Paulin, Lars ; Zhang, Xiaoping ; Gillis, Monique ; Lindstrom, Kristina. / Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya. In: International Journal of Systematic Bacteriology. 1999 ; Vol. 49, No. 4. pp. 1359-1368.
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Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya. / Nick, Giselle; De Lajudie, Philippe; Eardly, Bertrand D.; Suomalainen, Sini; Paulin, Lars; Zhang, Xiaoping; Gillis, Monique; Lindstrom, Kristina.

In: International Journal of Systematic Bacteriology, Vol. 49, No. 4, 01.01.1999, p. 1359-1368.

Research output: Contribution to journalArticle

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