SKP1 is involved in abscisic acid signalling to regulate seed germination, stomatal opening and root growth in Arabidopsis thaliana

Chijun Li, Zuojun Liu, Qirui Zhang, Ruozhong Wang, Langtao Xiao, Hong Ma, Kang Chong, Yunyuan Xu

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Abscisic acid (ABA) regulates many aspects of plant development, including seed dormancy and germination, root growth and stomatal closure. Plant SKP1 proteins are subunits of the SCF complex E3 ligases, which regulate several phytohormone signalling pathways through protein degradation. However, little is known about SKP1 proteins participating in ABA signalling. Here, we report that the overexpression of Triticum aestivum SKP1-like 1 (TSK1) in Arabidopsis thaliana (Arabidopsis) resulted in delayed seed germination and hypersensitivity to ABA. The opening of stomatal guard cells and the transcription of several ABA-responsive genes were affected in transgenic plants. In contrast, Arabidopsis skp1-like 1 (ask1)/ask1 ASK2/ask2 seedlings exhibited reduced ABA sensitivity. Furthermore, the transcription of ASK1 and ASK2 was down-regulated in abi1-1 and abi5-1 mutants compared with that in wild type. ASK1 or ASK2 overexpression could rescue or partially rescue the ABA insensitivity of abi5-1 mutants, respectively. Our work demonstrates that SKP1 is involved in ABA signalling and that SKP1-like genes may positively regulate ABA signalling by SCF-mediated protein degradation. SCF complex regulates several phytohormonal signalling pathways via a ubiquitination-mediated protein degradation. Here we report that transgenic Arabidopsis plant overexpressing Triticum aestivum SKP1-like 1 (TSK1) exhibited an ABA hypersensitive phenotype; whereas ask1/ask1 ASK2/ask2 mutant plants showed an opposite ABA response. These results suggest that SKP1-like genes may positively regulate ABA signalling through an SCF-mediated protein degradation process. The functional conservation and divergence of SKP1 genes are discussed.

Original languageEnglish (US)
Pages (from-to)952-965
Number of pages14
JournalPlant, Cell and Environment
Volume35
Issue number5
DOIs
StatePublished - May 2012

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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