Small heat shock proteins mediate cell-autonomous and -nonautonomous protection in a Drosophila model for environmental-stress-induced degeneration

Fumiko Kawasaki, Noelle L. Koonce, Linda Guo, Shahroz Fatima, Catherine Qiu, Mackenzie T. Moon, Yunzhen Zheng, Richard W. Ordway

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cell and tissue degeneration, and the development of degenerative diseases, are influenced by genetic and environmental factors that affect protein misfolding and proteotoxicity. To better understand the role of the environment in degeneration, we developed a genetic model for heat shock (HS)-stress-induced degeneration in Drosophila. This model exhibits a unique combination of features that enhance genetic analysis of degeneration and protection mechanisms involving environmental stress. These include celltype-specific failure of proteostasis and degeneration in response to global stress, cell-nonautonomous interactions within a simple and accessible network of susceptible cell types, and precise temporal control over the induction of degeneration. In wild-type flies, HS stress causes selective loss of the flight ability and degeneration of three susceptible cell types comprising the flight motor: muscle, motor neurons and associated glia. Other motor behaviors persist and, accordingly, the corresponding cell types controlling leg motor function are resistant to degeneration. Flight motor degeneration was preceded by a failure of muscle proteostasis characterized by diffuse ubiquitinated protein aggregates. Moreover, muscle-specific overexpression of a small heat shock protein (HSP), HSP23, promoted proteostasis and protected muscle from HS stress. Notably, neurons and glia were protected as well, indicating that a small HSP can mediate cell-nonautonomous protection. Cellautonomous protection of muscle was characterized by a distinct distribution of ubiquitinated proteins, including perinuclear localization and clearance of protein aggregates associated with the perinuclear microtubule network. This network was severely disrupted in wild-type preparations prior to degeneration, suggesting that it serves an important role in muscle proteostasis and protection. Finally, studies of resistant leg muscles revealed that they sustain proteostasis and the microtubule cytoskeleton after HS stress. These findings establish a model for genetic analysis of degeneration and protection mechanisms involving contributions of environmental factors, and advance our understanding of the protective functions and therapeutic potential of small HSPs.

Original languageEnglish (US)
Pages (from-to)953-964
Number of pages12
JournalDMM Disease Models and Mechanisms
Volume9
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Small Heat-Shock Proteins
Drosophila
Muscle
Muscles
Shock
Hot Temperature
Ubiquitinated Proteins
Genetic Models
Microtubules
Neuroglia
Neurons
Leg
Inborn Genetic Diseases
Aptitude
Cytoprotection
Motor Neurons
Cytoskeleton
Cell Communication
Diptera
Tissue

All Science Journal Classification (ASJC) codes

  • Neuroscience (miscellaneous)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kawasaki, Fumiko ; Koonce, Noelle L. ; Guo, Linda ; Fatima, Shahroz ; Qiu, Catherine ; Moon, Mackenzie T. ; Zheng, Yunzhen ; Ordway, Richard W. / Small heat shock proteins mediate cell-autonomous and -nonautonomous protection in a Drosophila model for environmental-stress-induced degeneration. In: DMM Disease Models and Mechanisms. 2016 ; Vol. 9, No. 9. pp. 953-964.
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Small heat shock proteins mediate cell-autonomous and -nonautonomous protection in a Drosophila model for environmental-stress-induced degeneration. / Kawasaki, Fumiko; Koonce, Noelle L.; Guo, Linda; Fatima, Shahroz; Qiu, Catherine; Moon, Mackenzie T.; Zheng, Yunzhen; Ordway, Richard W.

In: DMM Disease Models and Mechanisms, Vol. 9, No. 9, 01.09.2016, p. 953-964.

Research output: Contribution to journalArticle

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T1 - Small heat shock proteins mediate cell-autonomous and -nonautonomous protection in a Drosophila model for environmental-stress-induced degeneration

AU - Kawasaki, Fumiko

AU - Koonce, Noelle L.

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AU - Fatima, Shahroz

AU - Qiu, Catherine

AU - Moon, Mackenzie T.

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AU - Ordway, Richard W.

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AB - Cell and tissue degeneration, and the development of degenerative diseases, are influenced by genetic and environmental factors that affect protein misfolding and proteotoxicity. To better understand the role of the environment in degeneration, we developed a genetic model for heat shock (HS)-stress-induced degeneration in Drosophila. This model exhibits a unique combination of features that enhance genetic analysis of degeneration and protection mechanisms involving environmental stress. These include celltype-specific failure of proteostasis and degeneration in response to global stress, cell-nonautonomous interactions within a simple and accessible network of susceptible cell types, and precise temporal control over the induction of degeneration. In wild-type flies, HS stress causes selective loss of the flight ability and degeneration of three susceptible cell types comprising the flight motor: muscle, motor neurons and associated glia. Other motor behaviors persist and, accordingly, the corresponding cell types controlling leg motor function are resistant to degeneration. Flight motor degeneration was preceded by a failure of muscle proteostasis characterized by diffuse ubiquitinated protein aggregates. Moreover, muscle-specific overexpression of a small heat shock protein (HSP), HSP23, promoted proteostasis and protected muscle from HS stress. Notably, neurons and glia were protected as well, indicating that a small HSP can mediate cell-nonautonomous protection. Cellautonomous protection of muscle was characterized by a distinct distribution of ubiquitinated proteins, including perinuclear localization and clearance of protein aggregates associated with the perinuclear microtubule network. This network was severely disrupted in wild-type preparations prior to degeneration, suggesting that it serves an important role in muscle proteostasis and protection. Finally, studies of resistant leg muscles revealed that they sustain proteostasis and the microtubule cytoskeleton after HS stress. These findings establish a model for genetic analysis of degeneration and protection mechanisms involving contributions of environmental factors, and advance our understanding of the protective functions and therapeutic potential of small HSPs.

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