Sodium butyrate enhances fetal globin gene expression in erythroid progenitors of patients with Hb SS and β thalassemia

S. P. Perrine, Barbara Miller, D. V. Faller, R. A. Cohen, E. P. Vichinsky, D. Hurst, B. H. Lubin, T. Papayannopoulou

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Increasing the expression of the γ globin genes is considered a useful therapeutic approach to the β globin diseases. Because butyrate and α-amino-n-butyric acid (ABA) augment γ globin expression in normal neonatal and adult erythroid progenitors, we investigated the effects of sodium butyrate and ABA on erythroid progenitors of patients with β thalassemia and sickle cell anemia who might benefit from such an effect. Both substances increased fetal hemoglobin (Hb F) expression in Bfu-e from 7% to 30% above levels found in control cultures from the same subjects with sickle cell anemia. The fraction of cultured erythroblasts producing Hb F increased more than 20% with sodium butyrate treatment in 70% of cultures. In most cultures, this produced > 20% total Hb F and > 70% F cells, levels which have been considered beneficial in ameliorating clinical symptoms. Alpha: non-alpha (α-non-α) imbalance was decreased by 36% in erythroid progenitors of patients with β thalassemia cultured in the presence of butyrate compared with control cultures from the same subjects. These data suggest that sodium butyrate may have therapeutic potential for increasing γ globulin expression in the β globin diseases.

Original languageEnglish (US)
Pages (from-to)454-459
Number of pages6
JournalBlood
Volume74
Issue number1
StatePublished - Jan 1 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Perrine, S. P., Miller, B., Faller, D. V., Cohen, R. A., Vichinsky, E. P., Hurst, D., Lubin, B. H., & Papayannopoulou, T. (1989). Sodium butyrate enhances fetal globin gene expression in erythroid progenitors of patients with Hb SS and β thalassemia. Blood, 74(1), 454-459.