Soil microbiome transfer method affects microbiome composition, including dominant microorganisms, in a novel environment

Mia M. Howard, Terrence Bell, Jenny Kao-Kniffin

Research output: Contribution to journalLetter

10 Citations (Scopus)

Abstract

We show that choice of soil microbiome transfer method, i.e. direct soil transfers and a common soil wash procedure, dramatically influences the microbiome that develops in a new environment, using high-throughput amplicon sequencing of 16S rRNA genes and the fungal internal transcribed spacer (ITS) region. After 3 weeks of incubation in commercial potting mix, microbiomes were most similar to the source soil when a greater volume of initial soil was transferred (5% v/v transfer), and least similar when using a soil wash. Abundant operational taxonomic units were substantially affected by transfer method, suggesting that compounds transferred from the source soil, shifts in biotic interactions, or both, play an important role in their success.

Original languageEnglish (US)
Article numberfnx092
JournalFEMS Microbiology Letters
Volume364
Issue number11
DOIs
StatePublished - Jun 1 2017

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Microbiota
Soil
rRNA Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

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title = "Soil microbiome transfer method affects microbiome composition, including dominant microorganisms, in a novel environment",
abstract = "We show that choice of soil microbiome transfer method, i.e. direct soil transfers and a common soil wash procedure, dramatically influences the microbiome that develops in a new environment, using high-throughput amplicon sequencing of 16S rRNA genes and the fungal internal transcribed spacer (ITS) region. After 3 weeks of incubation in commercial potting mix, microbiomes were most similar to the source soil when a greater volume of initial soil was transferred (5{\%} v/v transfer), and least similar when using a soil wash. Abundant operational taxonomic units were substantially affected by transfer method, suggesting that compounds transferred from the source soil, shifts in biotic interactions, or both, play an important role in their success.",
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Soil microbiome transfer method affects microbiome composition, including dominant microorganisms, in a novel environment. / Howard, Mia M.; Bell, Terrence; Kao-Kniffin, Jenny.

In: FEMS Microbiology Letters, Vol. 364, No. 11, fnx092, 01.06.2017.

Research output: Contribution to journalLetter

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AB - We show that choice of soil microbiome transfer method, i.e. direct soil transfers and a common soil wash procedure, dramatically influences the microbiome that develops in a new environment, using high-throughput amplicon sequencing of 16S rRNA genes and the fungal internal transcribed spacer (ITS) region. After 3 weeks of incubation in commercial potting mix, microbiomes were most similar to the source soil when a greater volume of initial soil was transferred (5% v/v transfer), and least similar when using a soil wash. Abundant operational taxonomic units were substantially affected by transfer method, suggesting that compounds transferred from the source soil, shifts in biotic interactions, or both, play an important role in their success.

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