Solution structure of the (+)-cis-anti-Benzo[a]pyrene-da ([BP]dA) adduct opposite dT in a DNA duplex

Bing Mao, Zhentian Gu, Andrey Gorin, Junxin Chen, Brian E. Hingerty, Shantu Amin, Suse Broyde, Nicholas E. Geacintov, Dinshaw J. Patel

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Abstract

Minor adducts, derived from the covalent binding of anti-benzo[a]pyrene- 7,8-dihydroxy-9,10-epoxide to cellular DNA, may play an important role in generating mutations and initiating cancer. We have applied a combined NMR- computational approach including intensity based refinement to determine the solution structure of the minor (+)-cis-anti-[BP]dA adduct positioned opposite dT in the d(C1-T2-C3-T4-C5-[BP]A6-C7-T8-T9-C10-C11)·(d(G12-G13-A14- A15-G16-T17-G18A19-G20-A21-G22) 11-mer duplex. The BP ring system is intercalated toward the 5'-side of the [BP]dA6 lesion site without disrupting the flanking Watson-Crick dC5·dG18 and [BP]dA6·dT17 base pairs. This structure of the (+)-cis-anti-[BP]dA·dT 11-mer duplex, containing a bay region benzo[a]pyrenyl [BP]dA adduct, is compared with the corresponding structure of the (+)-trans-anti-[BPh]dA·dT 11-mer duplex (Cosman et al., Biochemistry 32, 12488-12497, 1993), which contains a fjord region benzo[c]phenanthrenyl [BPh]dA adduct with the same R stereochemistry at the linkage site. The carcinogen intercalates toward the 5'-direction of the modified strand in both duplexes (the adduct is embedded within the same sequence context) with the buckling of the Watson-Crick [BP]dA6·dT17 base pair more pronounced in the (+)-cis-anti-[BP]dA·dT 11-mer duplex compared to its Watson-Crick [BPh]dA·dT17 base pair in the (+)-trans-anti-[BPh]dA·dT 11-mer duplex. The available structural studies of covalent polycyclic aromatic hydrocarbon (PAH) carcinogen-DNA adducts point toward the emergence of a general theme where distinct alignments are adopted by PAH adducts covalently linked to the N6 of adenine when compared to the N2 of guanine in DNA duplexes. The [BPh]dA and [BP]dA N6-adenine adducts intercalate their polycyclic aromatic rings into the helix without disruption of their modified base pairs. This may reflect the potential flexibility associated with the positioning of the covalent tether and the benzylic ring of the carcinogen in the sterically spacious major groove. By contrast, such an intercalation without modified base pair disruption option appears not to be available to [BP]dG N2-guanine adducts where the Covalent tether and the benzylic ring are positioned in the more sterically crowded minor groove. In the case of [BP]dG adducts, the benzopyrenyl ring is either positioned in the minor groove without base pair disruption, or if intercalated into the helix, requires disruption of the modified base pair and displacement of the bases out of the helix.

Original languageEnglish (US)
Pages (from-to)10831-10842
Number of pages12
JournalBiochemistry
Volume38
Issue number33
DOIs
StatePublished - Aug 17 1999

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Benzo(a)pyrene
Base Pairing
Carcinogens
Polycyclic Aromatic Hydrocarbons
Guanine
Adenine
DNA
Biochemistry
Stereochemistry
DNA Adducts
Epoxy Compounds
Intercalation
Buckling
Nuclear magnetic resonance
Estuaries
Mutation
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Mao, B., Gu, Z., Gorin, A., Chen, J., Hingerty, B. E., Amin, S., ... Patel, D. J. (1999). Solution structure of the (+)-cis-anti-Benzo[a]pyrene-da ([BP]dA) adduct opposite dT in a DNA duplex. Biochemistry, 38(33), 10831-10842. https://doi.org/10.1021/bi991212f
Mao, Bing ; Gu, Zhentian ; Gorin, Andrey ; Chen, Junxin ; Hingerty, Brian E. ; Amin, Shantu ; Broyde, Suse ; Geacintov, Nicholas E. ; Patel, Dinshaw J. / Solution structure of the (+)-cis-anti-Benzo[a]pyrene-da ([BP]dA) adduct opposite dT in a DNA duplex. In: Biochemistry. 1999 ; Vol. 38, No. 33. pp. 10831-10842.
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abstract = "Minor adducts, derived from the covalent binding of anti-benzo[a]pyrene- 7,8-dihydroxy-9,10-epoxide to cellular DNA, may play an important role in generating mutations and initiating cancer. We have applied a combined NMR- computational approach including intensity based refinement to determine the solution structure of the minor (+)-cis-anti-[BP]dA adduct positioned opposite dT in the d(C1-T2-C3-T4-C5-[BP]A6-C7-T8-T9-C10-C11)·(d(G12-G13-A14- A15-G16-T17-G18A19-G20-A21-G22) 11-mer duplex. The BP ring system is intercalated toward the 5'-side of the [BP]dA6 lesion site without disrupting the flanking Watson-Crick dC5·dG18 and [BP]dA6·dT17 base pairs. This structure of the (+)-cis-anti-[BP]dA·dT 11-mer duplex, containing a bay region benzo[a]pyrenyl [BP]dA adduct, is compared with the corresponding structure of the (+)-trans-anti-[BPh]dA·dT 11-mer duplex (Cosman et al., Biochemistry 32, 12488-12497, 1993), which contains a fjord region benzo[c]phenanthrenyl [BPh]dA adduct with the same R stereochemistry at the linkage site. The carcinogen intercalates toward the 5'-direction of the modified strand in both duplexes (the adduct is embedded within the same sequence context) with the buckling of the Watson-Crick [BP]dA6·dT17 base pair more pronounced in the (+)-cis-anti-[BP]dA·dT 11-mer duplex compared to its Watson-Crick [BPh]dA·dT17 base pair in the (+)-trans-anti-[BPh]dA·dT 11-mer duplex. The available structural studies of covalent polycyclic aromatic hydrocarbon (PAH) carcinogen-DNA adducts point toward the emergence of a general theme where distinct alignments are adopted by PAH adducts covalently linked to the N6 of adenine when compared to the N2 of guanine in DNA duplexes. The [BPh]dA and [BP]dA N6-adenine adducts intercalate their polycyclic aromatic rings into the helix without disruption of their modified base pairs. This may reflect the potential flexibility associated with the positioning of the covalent tether and the benzylic ring of the carcinogen in the sterically spacious major groove. By contrast, such an intercalation without modified base pair disruption option appears not to be available to [BP]dG N2-guanine adducts where the Covalent tether and the benzylic ring are positioned in the more sterically crowded minor groove. In the case of [BP]dG adducts, the benzopyrenyl ring is either positioned in the minor groove without base pair disruption, or if intercalated into the helix, requires disruption of the modified base pair and displacement of the bases out of the helix.",
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Mao, B, Gu, Z, Gorin, A, Chen, J, Hingerty, BE, Amin, S, Broyde, S, Geacintov, NE & Patel, DJ 1999, 'Solution structure of the (+)-cis-anti-Benzo[a]pyrene-da ([BP]dA) adduct opposite dT in a DNA duplex', Biochemistry, vol. 38, no. 33, pp. 10831-10842. https://doi.org/10.1021/bi991212f

Solution structure of the (+)-cis-anti-Benzo[a]pyrene-da ([BP]dA) adduct opposite dT in a DNA duplex. / Mao, Bing; Gu, Zhentian; Gorin, Andrey; Chen, Junxin; Hingerty, Brian E.; Amin, Shantu; Broyde, Suse; Geacintov, Nicholas E.; Patel, Dinshaw J.

In: Biochemistry, Vol. 38, No. 33, 17.08.1999, p. 10831-10842.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Solution structure of the (+)-cis-anti-Benzo[a]pyrene-da ([BP]dA) adduct opposite dT in a DNA duplex

AU - Mao, Bing

AU - Gu, Zhentian

AU - Gorin, Andrey

AU - Chen, Junxin

AU - Hingerty, Brian E.

AU - Amin, Shantu

AU - Broyde, Suse

AU - Geacintov, Nicholas E.

AU - Patel, Dinshaw J.

PY - 1999/8/17

Y1 - 1999/8/17

N2 - Minor adducts, derived from the covalent binding of anti-benzo[a]pyrene- 7,8-dihydroxy-9,10-epoxide to cellular DNA, may play an important role in generating mutations and initiating cancer. We have applied a combined NMR- computational approach including intensity based refinement to determine the solution structure of the minor (+)-cis-anti-[BP]dA adduct positioned opposite dT in the d(C1-T2-C3-T4-C5-[BP]A6-C7-T8-T9-C10-C11)·(d(G12-G13-A14- A15-G16-T17-G18A19-G20-A21-G22) 11-mer duplex. The BP ring system is intercalated toward the 5'-side of the [BP]dA6 lesion site without disrupting the flanking Watson-Crick dC5·dG18 and [BP]dA6·dT17 base pairs. This structure of the (+)-cis-anti-[BP]dA·dT 11-mer duplex, containing a bay region benzo[a]pyrenyl [BP]dA adduct, is compared with the corresponding structure of the (+)-trans-anti-[BPh]dA·dT 11-mer duplex (Cosman et al., Biochemistry 32, 12488-12497, 1993), which contains a fjord region benzo[c]phenanthrenyl [BPh]dA adduct with the same R stereochemistry at the linkage site. The carcinogen intercalates toward the 5'-direction of the modified strand in both duplexes (the adduct is embedded within the same sequence context) with the buckling of the Watson-Crick [BP]dA6·dT17 base pair more pronounced in the (+)-cis-anti-[BP]dA·dT 11-mer duplex compared to its Watson-Crick [BPh]dA·dT17 base pair in the (+)-trans-anti-[BPh]dA·dT 11-mer duplex. The available structural studies of covalent polycyclic aromatic hydrocarbon (PAH) carcinogen-DNA adducts point toward the emergence of a general theme where distinct alignments are adopted by PAH adducts covalently linked to the N6 of adenine when compared to the N2 of guanine in DNA duplexes. The [BPh]dA and [BP]dA N6-adenine adducts intercalate their polycyclic aromatic rings into the helix without disruption of their modified base pairs. This may reflect the potential flexibility associated with the positioning of the covalent tether and the benzylic ring of the carcinogen in the sterically spacious major groove. By contrast, such an intercalation without modified base pair disruption option appears not to be available to [BP]dG N2-guanine adducts where the Covalent tether and the benzylic ring are positioned in the more sterically crowded minor groove. In the case of [BP]dG adducts, the benzopyrenyl ring is either positioned in the minor groove without base pair disruption, or if intercalated into the helix, requires disruption of the modified base pair and displacement of the bases out of the helix.

AB - Minor adducts, derived from the covalent binding of anti-benzo[a]pyrene- 7,8-dihydroxy-9,10-epoxide to cellular DNA, may play an important role in generating mutations and initiating cancer. We have applied a combined NMR- computational approach including intensity based refinement to determine the solution structure of the minor (+)-cis-anti-[BP]dA adduct positioned opposite dT in the d(C1-T2-C3-T4-C5-[BP]A6-C7-T8-T9-C10-C11)·(d(G12-G13-A14- A15-G16-T17-G18A19-G20-A21-G22) 11-mer duplex. The BP ring system is intercalated toward the 5'-side of the [BP]dA6 lesion site without disrupting the flanking Watson-Crick dC5·dG18 and [BP]dA6·dT17 base pairs. This structure of the (+)-cis-anti-[BP]dA·dT 11-mer duplex, containing a bay region benzo[a]pyrenyl [BP]dA adduct, is compared with the corresponding structure of the (+)-trans-anti-[BPh]dA·dT 11-mer duplex (Cosman et al., Biochemistry 32, 12488-12497, 1993), which contains a fjord region benzo[c]phenanthrenyl [BPh]dA adduct with the same R stereochemistry at the linkage site. The carcinogen intercalates toward the 5'-direction of the modified strand in both duplexes (the adduct is embedded within the same sequence context) with the buckling of the Watson-Crick [BP]dA6·dT17 base pair more pronounced in the (+)-cis-anti-[BP]dA·dT 11-mer duplex compared to its Watson-Crick [BPh]dA·dT17 base pair in the (+)-trans-anti-[BPh]dA·dT 11-mer duplex. The available structural studies of covalent polycyclic aromatic hydrocarbon (PAH) carcinogen-DNA adducts point toward the emergence of a general theme where distinct alignments are adopted by PAH adducts covalently linked to the N6 of adenine when compared to the N2 of guanine in DNA duplexes. The [BPh]dA and [BP]dA N6-adenine adducts intercalate their polycyclic aromatic rings into the helix without disruption of their modified base pairs. This may reflect the potential flexibility associated with the positioning of the covalent tether and the benzylic ring of the carcinogen in the sterically spacious major groove. By contrast, such an intercalation without modified base pair disruption option appears not to be available to [BP]dG N2-guanine adducts where the Covalent tether and the benzylic ring are positioned in the more sterically crowded minor groove. In the case of [BP]dG adducts, the benzopyrenyl ring is either positioned in the minor groove without base pair disruption, or if intercalated into the helix, requires disruption of the modified base pair and displacement of the bases out of the helix.

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