Solution structure of the natively assembled yeast ribosomal stalk etermined by small-angle X-ray scattering

Przemysław Grela, Michal J. Gajda, Jean Paul Armache, Roland Beckmann, Dawid Krokowski, Dmitri I. Svergun, Nikodem Grankowski, Marek Tchoŕzewski

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The ribosomal stalk of the 60S subunit has been shown to play a crucial role in all steps of protein synthesis, but its structure and exact molecular function remain an unanswered question. In the present study, we show the low-resolution models of the solution structure of the yeast ribosomal stalk, composed of five proteins, P0-(P1-P2)2. The model of the pentameric stalk complex determined by small-angle X-ray scattering reveals an elongated shape with a maximum length of 13 nm. The model displays three distinct lobes, which may correspond to the individual P1-P2 heterodimers anchored to the C-terminal domain of the P0 protein.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalBiochemical Journal
Volume444
Issue number2
DOIs
StatePublished - Jun 1 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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