Solution structure of the unbound, oxidized photosystem I subunit PsaC, containing [4Fe-4S] clusters FA and FB: A conformational change occurs upon binding to photosystem I

Mikhail L. Antonkine, Gaohua Liu, Detlef Bentrop, Donald Ashley Bryant, Ivano Bertini, Claudio Luchinat, John H. Golbeck, Dietmar Stehlik

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Abstract

This work presents the three-dimensional NMR solution structure of recombinant, oxidized, unbound PsaC from Synechococcus sp. PCC 7002. Constraints are derived from homo- and heteronuclear one-, two- and three-dimensional 1H and 15N NMR data. Significant differences are outlined between the unbound PsaC structure presented here and the available X-ray structure of bound PsaC as an integral part of the whole cyanobacterial PS I complex. These differences mainly concern the arrangement of the N- and C-termini with respect to the [4Fe-4S] core domain. In the NMR solution structure of PsaC the C-terminal region assumes a disordered helical conformation, and is clearly different from the extended coil conformation, which is one of the structural elements required to anchor PsaC to the PS I core heterodimer. In solution the N-terminus of PsaC is in contact with the pre-C-terminal region but slides in between the latter and the iron-sulfur core region of the protein. Together, these features result in a concerted movement of the N-terminus and pre-C-terminal region away from the FA binding site, accompanied by a bending of the N-terminus. In comparison, the same terminal regions are positioned much closer to FA and take up an anti-parallel β-sheet arrangement in PsaC bound to PS I. The conformational changes between bound and unbound PsaC correlate with the differences reported earlier for the EPR spectra of reduced FA and FB in bound versus unbound PsaC. The observed different structural features in solution are highly relevant for unraveling the stepwise assembly process of the stromal PsaC, PsaD and PsaE subunits to the PS I core heterodimer.

Original languageEnglish (US)
Pages (from-to)461-472
Number of pages12
JournalJournal of Biological Inorganic Chemistry
Volume7
Issue number4-5
DOIs
StatePublished - Dec 1 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

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