Solution 31Phosphorus Nuclear Magnetic Resonance Analysis of Acid-Extractable Phosphates in Animal Feces

C. Shang, T. H. Yang, R. O. Maguire, W. B. Staniar, K. F. Knowlton

Research output: Contribution to journalArticlepeer-review

Abstract

Characterizing phosphates in animal fecal and manure samples is of interest to environmental-monitoring research efforts. Acid extraction removes relatively mobile phosphates from samples, offering a better indicator of the mobility of phosphates in environment, but acidic extracts impose challenges to the solution phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy, which is commonly used for analyzing phosphates in environmental samples. Acid-extractable metals precipitate phosphates in the alkaline condition under which solution 31P NMR experiment runs, blurring the spectrum for adequate analysis. We found that neutralizing acid extracts with 0.5 M sodium hydroxide (NaOH) plus 50 mM ethylenediaminetetraacetic acid (EDTA) before freeze drying eliminated the chemical interferences otherwise observed. The resulting 31P NMR spectra can be used to quantify acid-soluble phytate and other phosphates in animal fecal and manure samples. The improvement in detection will support efforts to investigate the mobility of phosphates in feces and manures used as land amendments.

Original languageEnglish (US)
Pages (from-to)2353-2360
Number of pages8
JournalCommunications in Soil Science and Plant Analysis
Volume44
Issue number16
DOIs
StatePublished - Sep 2013

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Soil Science

Fingerprint Dive into the research topics of 'Solution <sup>31</sup>Phosphorus Nuclear Magnetic Resonance Analysis of Acid-Extractable Phosphates in Animal Feces'. Together they form a unique fingerprint.

Cite this