Solution 31Phosphorus Nuclear Magnetic Resonance Analysis of Acid-Extractable Phosphates in Animal Feces

C. Shang, T. H. Yang, R. O. Maguire, William Burton Staniar, K. F. Knowlton

Research output: Contribution to journalArticle

Abstract

Characterizing phosphates in animal fecal and manure samples is of interest to environmental-monitoring research efforts. Acid extraction removes relatively mobile phosphates from samples, offering a better indicator of the mobility of phosphates in environment, but acidic extracts impose challenges to the solution phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy, which is commonly used for analyzing phosphates in environmental samples. Acid-extractable metals precipitate phosphates in the alkaline condition under which solution 31P NMR experiment runs, blurring the spectrum for adequate analysis. We found that neutralizing acid extracts with 0.5 M sodium hydroxide (NaOH) plus 50 mM ethylenediaminetetraacetic acid (EDTA) before freeze drying eliminated the chemical interferences otherwise observed. The resulting 31P NMR spectra can be used to quantify acid-soluble phytate and other phosphates in animal fecal and manure samples. The improvement in detection will support efforts to investigate the mobility of phosphates in feces and manures used as land amendments.

Original languageEnglish (US)
Pages (from-to)2353-2360
Number of pages8
JournalCommunications in Soil Science and Plant Analysis
Volume44
Issue number16
DOIs
StatePublished - Sep 1 2013

Fingerprint

feces
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
phosphate
phosphates
animal
acids
acid
animals
animal manures
manure
freeze drying
sampling
environmental monitoring
sodium hydroxide
EDTA (chelating agent)
extracts
analysis
phytic acid
EDTA

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Soil Science

Cite this

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title = "Solution 31Phosphorus Nuclear Magnetic Resonance Analysis of Acid-Extractable Phosphates in Animal Feces",
abstract = "Characterizing phosphates in animal fecal and manure samples is of interest to environmental-monitoring research efforts. Acid extraction removes relatively mobile phosphates from samples, offering a better indicator of the mobility of phosphates in environment, but acidic extracts impose challenges to the solution phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy, which is commonly used for analyzing phosphates in environmental samples. Acid-extractable metals precipitate phosphates in the alkaline condition under which solution 31P NMR experiment runs, blurring the spectrum for adequate analysis. We found that neutralizing acid extracts with 0.5 M sodium hydroxide (NaOH) plus 50 mM ethylenediaminetetraacetic acid (EDTA) before freeze drying eliminated the chemical interferences otherwise observed. The resulting 31P NMR spectra can be used to quantify acid-soluble phytate and other phosphates in animal fecal and manure samples. The improvement in detection will support efforts to investigate the mobility of phosphates in feces and manures used as land amendments.",
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Solution 31Phosphorus Nuclear Magnetic Resonance Analysis of Acid-Extractable Phosphates in Animal Feces. / Shang, C.; Yang, T. H.; Maguire, R. O.; Staniar, William Burton; Knowlton, K. F.

In: Communications in Soil Science and Plant Analysis, Vol. 44, No. 16, 01.09.2013, p. 2353-2360.

Research output: Contribution to journalArticle

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