Somatic homologous recombination in planta: The recombination frequency is dependent on the allelic state of recombining sequences and may be influenced by genomic position effects

Peter Swoboda, Barbara Hohn, Susannah Gal

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have previously described a non-selective method for scoring somatic recombination in the genome of whole plants. The recombination substrate consists of a defective partial dimer of Cauliflower Mosaic Virus (CaMV) sequences, which can code for production of viable virus only upon homologous recombination; this leads to disease symptoms on leaves. Brassica napus plants (rapeseed) harbouring the recombination substrate as a transgene were used to examine the time in plant development at which recombination takes place. The analysis of three transgene loci revealed recombination frequencies specific for each locus. Recombination frequencies were increased if more than one transgene locus was present per genome, either in allelic (homozygosity of the transgene locus) or in non-allelic positions. In both cases, the overall recombination frequency was found to be elevated to approximately the sum of the frequencies for the individual transgene loci or slightly higher, suggesting that the respective transgene loci behave largely independently of each other. For all plants tested (single locus, two or multiple loci) maximal recombination frequencies were of the order of 10-6 events per cell division.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalMGG Molecular & General Genetics
Volume237
Issue number1-2
DOIs
StatePublished - Feb 1 1993

Fingerprint

Homologous Recombination
Genetic Recombination
Transgenes
Caulimovirus
Plant Genome
Brassica napus
Brassica rapa
Plant Development
Cell Division
Research Design
Genome
Viruses

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

@article{c9c279fa5f5e470a936250c2746d1fdf,
title = "Somatic homologous recombination in planta: The recombination frequency is dependent on the allelic state of recombining sequences and may be influenced by genomic position effects",
abstract = "We have previously described a non-selective method for scoring somatic recombination in the genome of whole plants. The recombination substrate consists of a defective partial dimer of Cauliflower Mosaic Virus (CaMV) sequences, which can code for production of viable virus only upon homologous recombination; this leads to disease symptoms on leaves. Brassica napus plants (rapeseed) harbouring the recombination substrate as a transgene were used to examine the time in plant development at which recombination takes place. The analysis of three transgene loci revealed recombination frequencies specific for each locus. Recombination frequencies were increased if more than one transgene locus was present per genome, either in allelic (homozygosity of the transgene locus) or in non-allelic positions. In both cases, the overall recombination frequency was found to be elevated to approximately the sum of the frequencies for the individual transgene loci or slightly higher, suggesting that the respective transgene loci behave largely independently of each other. For all plants tested (single locus, two or multiple loci) maximal recombination frequencies were of the order of 10-6 events per cell division.",
author = "Peter Swoboda and Barbara Hohn and Susannah Gal",
year = "1993",
month = "2",
day = "1",
doi = "10.1007/BF00282781",
language = "English (US)",
volume = "237",
pages = "33--40",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - Somatic homologous recombination in planta

T2 - The recombination frequency is dependent on the allelic state of recombining sequences and may be influenced by genomic position effects

AU - Swoboda, Peter

AU - Hohn, Barbara

AU - Gal, Susannah

PY - 1993/2/1

Y1 - 1993/2/1

N2 - We have previously described a non-selective method for scoring somatic recombination in the genome of whole plants. The recombination substrate consists of a defective partial dimer of Cauliflower Mosaic Virus (CaMV) sequences, which can code for production of viable virus only upon homologous recombination; this leads to disease symptoms on leaves. Brassica napus plants (rapeseed) harbouring the recombination substrate as a transgene were used to examine the time in plant development at which recombination takes place. The analysis of three transgene loci revealed recombination frequencies specific for each locus. Recombination frequencies were increased if more than one transgene locus was present per genome, either in allelic (homozygosity of the transgene locus) or in non-allelic positions. In both cases, the overall recombination frequency was found to be elevated to approximately the sum of the frequencies for the individual transgene loci or slightly higher, suggesting that the respective transgene loci behave largely independently of each other. For all plants tested (single locus, two or multiple loci) maximal recombination frequencies were of the order of 10-6 events per cell division.

AB - We have previously described a non-selective method for scoring somatic recombination in the genome of whole plants. The recombination substrate consists of a defective partial dimer of Cauliflower Mosaic Virus (CaMV) sequences, which can code for production of viable virus only upon homologous recombination; this leads to disease symptoms on leaves. Brassica napus plants (rapeseed) harbouring the recombination substrate as a transgene were used to examine the time in plant development at which recombination takes place. The analysis of three transgene loci revealed recombination frequencies specific for each locus. Recombination frequencies were increased if more than one transgene locus was present per genome, either in allelic (homozygosity of the transgene locus) or in non-allelic positions. In both cases, the overall recombination frequency was found to be elevated to approximately the sum of the frequencies for the individual transgene loci or slightly higher, suggesting that the respective transgene loci behave largely independently of each other. For all plants tested (single locus, two or multiple loci) maximal recombination frequencies were of the order of 10-6 events per cell division.

UR - http://www.scopus.com/inward/record.url?scp=0027399031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027399031&partnerID=8YFLogxK

U2 - 10.1007/BF00282781

DO - 10.1007/BF00282781

M3 - Article

C2 - 8455565

AN - SCOPUS:0027399031

VL - 237

SP - 33

EP - 40

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 1-2

ER -