Sophora microphylla (fabaceae) microsatellite markers and their utility across the genus

Megan L. Van Etten, Gary J. Houliston, Caroline M. Mitchell, Peter B. Heenan, Alastair W. Robertson, Jennifer A. Tate

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Premise of the study: Genus-specifi c microsatellite markers were developed for Sophora for population genetic and systematic studies of the group in New Zealand, and potentially elsewhere in the geographic range. Methods and Results: From sequencing a totalgenomic DNA library (using Roche 454), we identifi ed and developed 29 polymorphic microsatellite markers for S. microphylla and S. chathamica . We tested 12 of these markers on 14 S. chathamica individuals and four S. microphylla populations. Allloci amplifi ed in both species and species-specifi c alleles occurred at seven loci. In S. microphylla populations, the observed and expected heterozygosities ranged from 0.000-0.960 and 0.000-0.908, respectively, with alleles per locus ranging from seven to 23. Conclusions: The developed markers willbe valuable in studies of phylogenetics, population structure, mating system, and selection of provenances for restoration projects.

Original languageEnglish (US)
Article number1300081
JournalApplications in Plant Sciences
Volume2
Issue number3
DOIs
StatePublished - Mar 2014

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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