Soy protein concentrate mitigates markers of colonic inflammation and loss of gut barrier function in vitro and in vivo

Zachary T. Bitzer, Amy L. Wopperer, Benjamin J. Chrisfield, Ling Tao, Timothy Cooper, Jairam K.P. Vanamala, Ryan Elias, John E. Hayes, Joshua D. Lambert

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from H2O2-induced cell death and mitigated intracellular oxidative stress. Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases in monolayer permeability. Pepsin/pancreatin-digested SPC had reduced radical scavenging activity, but retained the monolayer protective effects of SPC. In vivo, 1.5% DSS caused body weight loss, colon shortening, and splenomegaly in CF-1 mice. Co-treatment with 12% SPC mitigated DSS-induced body weight loss and splenomegaly. DSS increased colonic interleukin (IL)-1β, IL-6, and monocyte chemotactic protein-1 expression. The levels of these markers were significantly lower in mice co-treated with SPC. SPC prevented DSS-mediated reductions in colonic glucagon-like peptide 2 levels, suggesting that SPC can prevent loss of gut barrier function, but no significant effect on claudin 1 and occludin mRNA levels of was observed. SPC-treated mice had lower colonic mRNA expression of toll-like receptor 4 and nucleotide-binding oligomerization domain-containing protein-like receptor family, pyrin domain containing protein 3 (NLRP3), and lower caspase-1 enzyme activity than DSS-treated mice. In summary, SPC exerted antioxidant and cytoprotective effects in vitro and moderated the severity of DSS-induced inflammation and loss of gut barrier function in vivo. These effects appear to be mediated in part through reduced NLRP3 expression and caspase 1 activity.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume40
DOIs
StatePublished - Feb 1 2017

Fingerprint

Soybean Proteins
Dextran Sulfate
Inflammation
Caspase 1
Splenomegaly
Scavenging
In Vitro Techniques
Weight Loss
Monolayers
Colon
Glucagon-Like Peptide 2
Claudin-1
Body Weight
Pancreatin
Occludin
Oligomerization
Messenger RNA
Toll-Like Receptor 4
Isoflavones
Oxidative stress

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Bitzer, Zachary T. ; Wopperer, Amy L. ; Chrisfield, Benjamin J. ; Tao, Ling ; Cooper, Timothy ; Vanamala, Jairam K.P. ; Elias, Ryan ; Hayes, John E. ; Lambert, Joshua D. / Soy protein concentrate mitigates markers of colonic inflammation and loss of gut barrier function in vitro and in vivo. In: Journal of Nutritional Biochemistry. 2017 ; Vol. 40. pp. 201-208.
@article{3d7a0e5010024739999be683ad010920,
title = "Soy protein concentrate mitigates markers of colonic inflammation and loss of gut barrier function in vitro and in vivo",
abstract = "Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from H2O2-induced cell death and mitigated intracellular oxidative stress. Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases in monolayer permeability. Pepsin/pancreatin-digested SPC had reduced radical scavenging activity, but retained the monolayer protective effects of SPC. In vivo, 1.5{\%} DSS caused body weight loss, colon shortening, and splenomegaly in CF-1 mice. Co-treatment with 12{\%} SPC mitigated DSS-induced body weight loss and splenomegaly. DSS increased colonic interleukin (IL)-1β, IL-6, and monocyte chemotactic protein-1 expression. The levels of these markers were significantly lower in mice co-treated with SPC. SPC prevented DSS-mediated reductions in colonic glucagon-like peptide 2 levels, suggesting that SPC can prevent loss of gut barrier function, but no significant effect on claudin 1 and occludin mRNA levels of was observed. SPC-treated mice had lower colonic mRNA expression of toll-like receptor 4 and nucleotide-binding oligomerization domain-containing protein-like receptor family, pyrin domain containing protein 3 (NLRP3), and lower caspase-1 enzyme activity than DSS-treated mice. In summary, SPC exerted antioxidant and cytoprotective effects in vitro and moderated the severity of DSS-induced inflammation and loss of gut barrier function in vivo. These effects appear to be mediated in part through reduced NLRP3 expression and caspase 1 activity.",
author = "Bitzer, {Zachary T.} and Wopperer, {Amy L.} and Chrisfield, {Benjamin J.} and Ling Tao and Timothy Cooper and Vanamala, {Jairam K.P.} and Ryan Elias and Hayes, {John E.} and Lambert, {Joshua D.}",
year = "2017",
month = "2",
day = "1",
doi = "10.1016/j.jnutbio.2016.11.012",
language = "English (US)",
volume = "40",
pages = "201--208",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier Inc.",

}

Soy protein concentrate mitigates markers of colonic inflammation and loss of gut barrier function in vitro and in vivo. / Bitzer, Zachary T.; Wopperer, Amy L.; Chrisfield, Benjamin J.; Tao, Ling; Cooper, Timothy; Vanamala, Jairam K.P.; Elias, Ryan; Hayes, John E.; Lambert, Joshua D.

In: Journal of Nutritional Biochemistry, Vol. 40, 01.02.2017, p. 201-208.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Soy protein concentrate mitigates markers of colonic inflammation and loss of gut barrier function in vitro and in vivo

AU - Bitzer, Zachary T.

AU - Wopperer, Amy L.

AU - Chrisfield, Benjamin J.

AU - Tao, Ling

AU - Cooper, Timothy

AU - Vanamala, Jairam K.P.

AU - Elias, Ryan

AU - Hayes, John E.

AU - Lambert, Joshua D.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from H2O2-induced cell death and mitigated intracellular oxidative stress. Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases in monolayer permeability. Pepsin/pancreatin-digested SPC had reduced radical scavenging activity, but retained the monolayer protective effects of SPC. In vivo, 1.5% DSS caused body weight loss, colon shortening, and splenomegaly in CF-1 mice. Co-treatment with 12% SPC mitigated DSS-induced body weight loss and splenomegaly. DSS increased colonic interleukin (IL)-1β, IL-6, and monocyte chemotactic protein-1 expression. The levels of these markers were significantly lower in mice co-treated with SPC. SPC prevented DSS-mediated reductions in colonic glucagon-like peptide 2 levels, suggesting that SPC can prevent loss of gut barrier function, but no significant effect on claudin 1 and occludin mRNA levels of was observed. SPC-treated mice had lower colonic mRNA expression of toll-like receptor 4 and nucleotide-binding oligomerization domain-containing protein-like receptor family, pyrin domain containing protein 3 (NLRP3), and lower caspase-1 enzyme activity than DSS-treated mice. In summary, SPC exerted antioxidant and cytoprotective effects in vitro and moderated the severity of DSS-induced inflammation and loss of gut barrier function in vivo. These effects appear to be mediated in part through reduced NLRP3 expression and caspase 1 activity.

AB - Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SPC protected Caco-2 human colon cells from H2O2-induced cell death and mitigated intracellular oxidative stress. Treatment of differentiated Caco-2 cells with SPC blunted DSS-induced increases in monolayer permeability. Pepsin/pancreatin-digested SPC had reduced radical scavenging activity, but retained the monolayer protective effects of SPC. In vivo, 1.5% DSS caused body weight loss, colon shortening, and splenomegaly in CF-1 mice. Co-treatment with 12% SPC mitigated DSS-induced body weight loss and splenomegaly. DSS increased colonic interleukin (IL)-1β, IL-6, and monocyte chemotactic protein-1 expression. The levels of these markers were significantly lower in mice co-treated with SPC. SPC prevented DSS-mediated reductions in colonic glucagon-like peptide 2 levels, suggesting that SPC can prevent loss of gut barrier function, but no significant effect on claudin 1 and occludin mRNA levels of was observed. SPC-treated mice had lower colonic mRNA expression of toll-like receptor 4 and nucleotide-binding oligomerization domain-containing protein-like receptor family, pyrin domain containing protein 3 (NLRP3), and lower caspase-1 enzyme activity than DSS-treated mice. In summary, SPC exerted antioxidant and cytoprotective effects in vitro and moderated the severity of DSS-induced inflammation and loss of gut barrier function in vivo. These effects appear to be mediated in part through reduced NLRP3 expression and caspase 1 activity.

UR - http://www.scopus.com/inward/record.url?scp=85007210853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007210853&partnerID=8YFLogxK

U2 - 10.1016/j.jnutbio.2016.11.012

DO - 10.1016/j.jnutbio.2016.11.012

M3 - Article

C2 - 27951472

AN - SCOPUS:85007210853

VL - 40

SP - 201

EP - 208

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

ER -