Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes

Daisuke Satoh, Daichi Sato, Taiichi Tsuyama, Motoki Saito, Hiroyuki Ohkura, Melissa Rolls, Fuyuki Ishikawa, Tadashi Uemura

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Dendrites allow neurons to integrate sensory or synaptic inputs, and the spatial disposition and local density of branches within the dendritic arbor limit the number and type of inputs. Drosophila melanogaster dendritic arborization (da) neurons provide a model system to study the genetic programs underlying such geometry in vivo. Here we report that mutations of motor-protein genes, including a dynein subunit gene (dlic) and kinesin heavy chain (khc), caused not only downsizing of the overall arbor, but also a marked shift of branching activity to the proximal area within the arbor. This phenotype was suppressed when dominant-negative Rab5 was expressed in the mutant neurons, which deposited early endosomes in the cell body. We also showed that 1) in dendritic branches of the wild-type neurons, Rab5-containing early endosomes were dynamically transported and 2) when Rab5 function alone was abrogated, terminal branches were almost totally deleted. These results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis.

Original languageEnglish (US)
Pages (from-to)1164-1171
Number of pages8
JournalNature Cell Biology
Volume10
Issue number10
DOIs
StatePublished - Sep 8 2008

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Dyneins
Endosomes
Neurons
Dendrites
Kinesin
Neuronal Plasticity
Drosophila melanogaster
Morphogenesis
Microtubules
Phenotype
Mutation
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Satoh, Daisuke ; Sato, Daichi ; Tsuyama, Taiichi ; Saito, Motoki ; Ohkura, Hiroyuki ; Rolls, Melissa ; Ishikawa, Fuyuki ; Uemura, Tadashi. / Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes. In: Nature Cell Biology. 2008 ; Vol. 10, No. 10. pp. 1164-1171.
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Satoh, D, Sato, D, Tsuyama, T, Saito, M, Ohkura, H, Rolls, M, Ishikawa, F & Uemura, T 2008, 'Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes', Nature Cell Biology, vol. 10, no. 10, pp. 1164-1171. https://doi.org/10.1038/ncb1776

Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes. / Satoh, Daisuke; Sato, Daichi; Tsuyama, Taiichi; Saito, Motoki; Ohkura, Hiroyuki; Rolls, Melissa; Ishikawa, Fuyuki; Uemura, Tadashi.

In: Nature Cell Biology, Vol. 10, No. 10, 08.09.2008, p. 1164-1171.

Research output: Contribution to journalArticle

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