Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan

Muhammad Asad Ali, Khushi Muhammad, Masood Rabbani, Aftab Ahmad Anjum, Mansur Ud Din Ahmad, Muhammad Zubair Shabbir, Muhammad Hamid Chaudhry, Bhushan M. Jayarao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Burkholderia mallei, a zoonotic pathogen, causes glanders in equines. The equids acquire infection from contaminated soil, water and excretions. A study was conducted to determine the geo-spatial distribution of B. mallei genome in soil samples of 10% percent villages (n=456) from eight districts of Punjab province, Pakistan by real time Polymerase chain reaction. Soil samples (n=2,280) from spatially distributed positive localities for B. mallei genome were maped in relation to canals, rivers, roads and railway lines. Real time-PCR was used for detection of B. mallei genome from soil samples wth positive control (118 copies/reaction) of the DNA. Eleven (0.48%) out of 2, 280 soil samples were positive for B. mallei genome distributed in varied locations of Punjab. The samples collected from Sheikhupura district showed higher prevalence (2.37%) followed by Chakwal district (2.10%). None of the samples from Gujranwala, Sahiwal, DG Khan, Attock, Faisalabad and Sargodha districts were found positive for B. mallei genome. The genome of B. mallei was distributed in 25% study districts of Punjab, Pakistan. Findings of the study suggested that RT-PCR assay could be used for detection of B. mallei in clinical samples.

Original languageEnglish (US)
Title of host publicationProceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
EditorsMuhammad Zafar-uz-Zaman
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages197-213
Number of pages17
ISBN (Electronic)9781467390736
DOIs
StatePublished - Mar 1 2017
Event14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 - Islamabad, Pakistan
Duration: Jan 10 2017Jan 14 2017

Publication series

NameProceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017

Other

Other14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
CountryPakistan
CityIslamabad
Period1/10/171/14/17

Fingerprint

Spatial distribution
Genes
Soils
Polymerase chain reaction
Pathogens
Canals
Assays
DNA
Rivers
Water

All Science Journal Classification (ASJC) codes

  • Computer Science (miscellaneous)

Cite this

Ali, M. A., Muhammad, K., Rabbani, M., Anjum, A. A., Ahmad, M. U. D., Shabbir, M. Z., ... Jayarao, B. M. (2017). Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan. In M. Zafar-uz-Zaman (Ed.), Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017 (pp. 197-213). [7868056] (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IBCAST.2017.7868056
Ali, Muhammad Asad ; Muhammad, Khushi ; Rabbani, Masood ; Anjum, Aftab Ahmad ; Ahmad, Mansur Ud Din ; Shabbir, Muhammad Zubair ; Chaudhry, Muhammad Hamid ; Jayarao, Bhushan M. / Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan. Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017. editor / Muhammad Zafar-uz-Zaman. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 197-213 (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017).
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abstract = "Burkholderia mallei, a zoonotic pathogen, causes glanders in equines. The equids acquire infection from contaminated soil, water and excretions. A study was conducted to determine the geo-spatial distribution of B. mallei genome in soil samples of 10{\%} percent villages (n=456) from eight districts of Punjab province, Pakistan by real time Polymerase chain reaction. Soil samples (n=2,280) from spatially distributed positive localities for B. mallei genome were maped in relation to canals, rivers, roads and railway lines. Real time-PCR was used for detection of B. mallei genome from soil samples wth positive control (118 copies/reaction) of the DNA. Eleven (0.48{\%}) out of 2, 280 soil samples were positive for B. mallei genome distributed in varied locations of Punjab. The samples collected from Sheikhupura district showed higher prevalence (2.37{\%}) followed by Chakwal district (2.10{\%}). None of the samples from Gujranwala, Sahiwal, DG Khan, Attock, Faisalabad and Sargodha districts were found positive for B. mallei genome. The genome of B. mallei was distributed in 25{\%} study districts of Punjab, Pakistan. Findings of the study suggested that RT-PCR assay could be used for detection of B. mallei in clinical samples.",
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Ali, MA, Muhammad, K, Rabbani, M, Anjum, AA, Ahmad, MUD, Shabbir, MZ, Chaudhry, MH & Jayarao, BM 2017, Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan. in M Zafar-uz-Zaman (ed.), Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017., 7868056, Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017, Institute of Electrical and Electronics Engineers Inc., pp. 197-213, 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017, Islamabad, Pakistan, 1/10/17. https://doi.org/10.1109/IBCAST.2017.7868056

Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan. / Ali, Muhammad Asad; Muhammad, Khushi; Rabbani, Masood; Anjum, Aftab Ahmad; Ahmad, Mansur Ud Din; Shabbir, Muhammad Zubair; Chaudhry, Muhammad Hamid; Jayarao, Bhushan M.

Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017. ed. / Muhammad Zafar-uz-Zaman. Institute of Electrical and Electronics Engineers Inc., 2017. p. 197-213 7868056 (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan

AU - Ali, Muhammad Asad

AU - Muhammad, Khushi

AU - Rabbani, Masood

AU - Anjum, Aftab Ahmad

AU - Ahmad, Mansur Ud Din

AU - Shabbir, Muhammad Zubair

AU - Chaudhry, Muhammad Hamid

AU - Jayarao, Bhushan M.

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AB - Burkholderia mallei, a zoonotic pathogen, causes glanders in equines. The equids acquire infection from contaminated soil, water and excretions. A study was conducted to determine the geo-spatial distribution of B. mallei genome in soil samples of 10% percent villages (n=456) from eight districts of Punjab province, Pakistan by real time Polymerase chain reaction. Soil samples (n=2,280) from spatially distributed positive localities for B. mallei genome were maped in relation to canals, rivers, roads and railway lines. Real time-PCR was used for detection of B. mallei genome from soil samples wth positive control (118 copies/reaction) of the DNA. Eleven (0.48%) out of 2, 280 soil samples were positive for B. mallei genome distributed in varied locations of Punjab. The samples collected from Sheikhupura district showed higher prevalence (2.37%) followed by Chakwal district (2.10%). None of the samples from Gujranwala, Sahiwal, DG Khan, Attock, Faisalabad and Sargodha districts were found positive for B. mallei genome. The genome of B. mallei was distributed in 25% study districts of Punjab, Pakistan. Findings of the study suggested that RT-PCR assay could be used for detection of B. mallei in clinical samples.

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Ali MA, Muhammad K, Rabbani M, Anjum AA, Ahmad MUD, Shabbir MZ et al. Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan. In Zafar-uz-Zaman M, editor, Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 197-213. 7868056. (Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017). https://doi.org/10.1109/IBCAST.2017.7868056