Spatial mapping of membrane phases in confluent endothelial cells

Peter J. Butler, Benjamin P. Bowen, Neal Woodbury

Research output: Contribution to journalConference article

Abstract

Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to blood borne molecules and hemodynamic forces which exact potent changes in EC biology. The EC plasma membrane transduces molecular and mechanical signals through membrane proteins which are sensitive to the lipid phase state surrounding them. We describe a method for high spatial resolution mapping of membrane phase states using molecular spectroscopic measurements of fluorescence lifetimes and polarizations of phase-specific lipoid dyes. The role of membrane phases in signal transduction is discussed.

Original languageEnglish (US)
Pages (from-to)582-583
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - Dec 1 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Fingerprint

Endothelial cells
Endothelial Cells
Cell membranes
Membranes
Blood
Cell Membrane
Cytology
Signal transduction
Fluorescence Polarization
Hemodynamics
Linings
Lipids
Cell Biology
Signal Transduction
Membrane Proteins
Coloring Agents
Dyes
Fluorescence
Polarization
Proteins

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

@article{333b1a74c2d6444a973ced65a7832a64,
title = "Spatial mapping of membrane phases in confluent endothelial cells",
abstract = "Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to blood borne molecules and hemodynamic forces which exact potent changes in EC biology. The EC plasma membrane transduces molecular and mechanical signals through membrane proteins which are sensitive to the lipid phase state surrounding them. We describe a method for high spatial resolution mapping of membrane phase states using molecular spectroscopic measurements of fluorescence lifetimes and polarizations of phase-specific lipoid dyes. The role of membrane phases in signal transduction is discussed.",
author = "Butler, {Peter J.} and Bowen, {Benjamin P.} and Neal Woodbury",
year = "2002",
month = "12",
day = "1",
language = "English (US)",
volume = "1",
pages = "582--583",
journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",
issn = "1557-170X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

Spatial mapping of membrane phases in confluent endothelial cells. / Butler, Peter J.; Bowen, Benjamin P.; Woodbury, Neal.

In: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, Vol. 1, 01.12.2002, p. 582-583.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Spatial mapping of membrane phases in confluent endothelial cells

AU - Butler, Peter J.

AU - Bowen, Benjamin P.

AU - Woodbury, Neal

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to blood borne molecules and hemodynamic forces which exact potent changes in EC biology. The EC plasma membrane transduces molecular and mechanical signals through membrane proteins which are sensitive to the lipid phase state surrounding them. We describe a method for high spatial resolution mapping of membrane phase states using molecular spectroscopic measurements of fluorescence lifetimes and polarizations of phase-specific lipoid dyes. The role of membrane phases in signal transduction is discussed.

AB - Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to blood borne molecules and hemodynamic forces which exact potent changes in EC biology. The EC plasma membrane transduces molecular and mechanical signals through membrane proteins which are sensitive to the lipid phase state surrounding them. We describe a method for high spatial resolution mapping of membrane phase states using molecular spectroscopic measurements of fluorescence lifetimes and polarizations of phase-specific lipoid dyes. The role of membrane phases in signal transduction is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0036916845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036916845&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0036916845

VL - 1

SP - 582

EP - 583

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

JF - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

SN - 1557-170X

ER -