Spatial mapping of membrane phases in confluent endothelial cells

Peter J. Butler, Benjamin P. Bowen, Neal Woodbury

Research output: Contribution to journalConference article

Abstract

Endothelial cells (ECs) form the inner lining of the blood vasculature and are therefore exposed to blood borne molecules and hemodynamic forces which exact potent changes in EC biology. The EC plasma membrane transduces molecular and mechanical signals through membrane proteins which are sensitive to the lipid phase state surrounding them. We describe a method for high spatial resolution mapping of membrane phase states using molecular spectroscopic measurements of fluorescence lifetimes and polarizations of phase-specific lipoid dyes. The role of membrane phases in signal transduction is discussed.

Original languageEnglish (US)
Pages (from-to)582-583
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - Dec 1 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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