Spatial resolution of exocytosis across a single cell by a microwell-based individually addressable thin film ultra-microelectrode array

Jun Wang, Raphaël Trouillon, Johan Dunevall, Andrew G. Ewing

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the fabrication and characterization of micro well-based individually addressable ultra-microelectrode arrays (MEAs) and their application to spatially and temporally resolved detection of neurotransmitter release across a single pheochromocytoma (PC 12) cell. The MEAs consist of sixteen 4-μm-width square microelectrodes, or twenty-five 3-μm-width square microelectrodes, or thirty-six 2-jim-width square microelectrodes. Each MEA is tightly defined in a 30×30 μm square area, which is further encased inside a 40×40 μm SU-8 microwell. We demonstrate the excellent stability and reproducibility of these microelectrodes by using cyclic voltammetry and we have performed recording of spatially resolution of single cell exocytosis with multiple ultra-microelectrodes in 2-μm resolution.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages392-394
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

All Science Journal Classification (ASJC) codes

  • Bioengineering

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