Specialist and generalist fungal parasites induce distinct biochemical changes in the mandible muscles of their host

Shanshan Zheng, Raquel Loreto, Philip Smith, Andrew Patterson, David Hughes, Liande Wang

Research output: Contribution to journalArticle

Abstract

Some parasites have evolved the ability to adaptively manipulate host behavior. One notable example is the fungus Ophiocordyceps unilateralis sensu lato, which has evolved the ability to alter the behavior of ants in ways that enable fungal transmission and lifecycle completion. Because host mandibles are affected by the fungi, wefocused on understanding changes in the metabolites of muscles during behavioral modification. We used High-Performance Liquid Chromatography-Mass/Mass (HPLC-MS/MS) to detect the metabolite difference between controls and O. unilateralis-infected ants. There was a significant difference between the global metabolome of O. unilateralis-infected ants and healthy ants, while there was no significant difference between the Beauveria bassiana treatment ants group compared to the healthy ants. A total of 31 and 16 of metabolites were putatively identified from comparisons of healthy ants with O. unilateralis-infected ants and comparisons of B. bassiana with O. unilateralis-infected samples, respectively. This result indicates that the concentrations of sugars, purines, ergothioneine, and hypoxanthine were significantly increased in O. unilateralis-infected ants in comparison to healthy ants and B. bassiana-infected ants. This study provides a comprehensive metabolic approach for understanding the interactions, at the level of host muscles, between healthy ants and fungal parasites.

Original languageEnglish (US)
Article number4589
JournalInternational journal of molecular sciences
Volume20
Issue number18
DOIs
StatePublished - Sep 2 2019

Fingerprint

parasites
Ants
metabolites
Metabolites
muscles
Mandible
Muscle
Parasites
fungi
Fungi
Muscles
Ergothioneine
Purines
purines
Hypoxanthine
liquid chromatography
Beauveria
High performance liquid chromatography
sugars
Sugars

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

@article{cac703e13aac4d8cab7c6790ced1a967,
title = "Specialist and generalist fungal parasites induce distinct biochemical changes in the mandible muscles of their host",
abstract = "Some parasites have evolved the ability to adaptively manipulate host behavior. One notable example is the fungus Ophiocordyceps unilateralis sensu lato, which has evolved the ability to alter the behavior of ants in ways that enable fungal transmission and lifecycle completion. Because host mandibles are affected by the fungi, wefocused on understanding changes in the metabolites of muscles during behavioral modification. We used High-Performance Liquid Chromatography-Mass/Mass (HPLC-MS/MS) to detect the metabolite difference between controls and O. unilateralis-infected ants. There was a significant difference between the global metabolome of O. unilateralis-infected ants and healthy ants, while there was no significant difference between the Beauveria bassiana treatment ants group compared to the healthy ants. A total of 31 and 16 of metabolites were putatively identified from comparisons of healthy ants with O. unilateralis-infected ants and comparisons of B. bassiana with O. unilateralis-infected samples, respectively. This result indicates that the concentrations of sugars, purines, ergothioneine, and hypoxanthine were significantly increased in O. unilateralis-infected ants in comparison to healthy ants and B. bassiana-infected ants. This study provides a comprehensive metabolic approach for understanding the interactions, at the level of host muscles, between healthy ants and fungal parasites.",
author = "Shanshan Zheng and Raquel Loreto and Philip Smith and Andrew Patterson and David Hughes and Liande Wang",
year = "2019",
month = "9",
day = "2",
doi = "10.3390/ijms20184589",
language = "English (US)",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "18",

}

Specialist and generalist fungal parasites induce distinct biochemical changes in the mandible muscles of their host. / Zheng, Shanshan; Loreto, Raquel; Smith, Philip; Patterson, Andrew; Hughes, David; Wang, Liande.

In: International journal of molecular sciences, Vol. 20, No. 18, 4589, 02.09.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Specialist and generalist fungal parasites induce distinct biochemical changes in the mandible muscles of their host

AU - Zheng, Shanshan

AU - Loreto, Raquel

AU - Smith, Philip

AU - Patterson, Andrew

AU - Hughes, David

AU - Wang, Liande

PY - 2019/9/2

Y1 - 2019/9/2

N2 - Some parasites have evolved the ability to adaptively manipulate host behavior. One notable example is the fungus Ophiocordyceps unilateralis sensu lato, which has evolved the ability to alter the behavior of ants in ways that enable fungal transmission and lifecycle completion. Because host mandibles are affected by the fungi, wefocused on understanding changes in the metabolites of muscles during behavioral modification. We used High-Performance Liquid Chromatography-Mass/Mass (HPLC-MS/MS) to detect the metabolite difference between controls and O. unilateralis-infected ants. There was a significant difference between the global metabolome of O. unilateralis-infected ants and healthy ants, while there was no significant difference between the Beauveria bassiana treatment ants group compared to the healthy ants. A total of 31 and 16 of metabolites were putatively identified from comparisons of healthy ants with O. unilateralis-infected ants and comparisons of B. bassiana with O. unilateralis-infected samples, respectively. This result indicates that the concentrations of sugars, purines, ergothioneine, and hypoxanthine were significantly increased in O. unilateralis-infected ants in comparison to healthy ants and B. bassiana-infected ants. This study provides a comprehensive metabolic approach for understanding the interactions, at the level of host muscles, between healthy ants and fungal parasites.

AB - Some parasites have evolved the ability to adaptively manipulate host behavior. One notable example is the fungus Ophiocordyceps unilateralis sensu lato, which has evolved the ability to alter the behavior of ants in ways that enable fungal transmission and lifecycle completion. Because host mandibles are affected by the fungi, wefocused on understanding changes in the metabolites of muscles during behavioral modification. We used High-Performance Liquid Chromatography-Mass/Mass (HPLC-MS/MS) to detect the metabolite difference between controls and O. unilateralis-infected ants. There was a significant difference between the global metabolome of O. unilateralis-infected ants and healthy ants, while there was no significant difference between the Beauveria bassiana treatment ants group compared to the healthy ants. A total of 31 and 16 of metabolites were putatively identified from comparisons of healthy ants with O. unilateralis-infected ants and comparisons of B. bassiana with O. unilateralis-infected samples, respectively. This result indicates that the concentrations of sugars, purines, ergothioneine, and hypoxanthine were significantly increased in O. unilateralis-infected ants in comparison to healthy ants and B. bassiana-infected ants. This study provides a comprehensive metabolic approach for understanding the interactions, at the level of host muscles, between healthy ants and fungal parasites.

UR - http://www.scopus.com/inward/record.url?scp=85072388159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072388159&partnerID=8YFLogxK

U2 - 10.3390/ijms20184589

DO - 10.3390/ijms20184589

M3 - Article

C2 - 31533250

AN - SCOPUS:85072388159

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 18

M1 - 4589

ER -