Species-specificity of estradiol regulated growth factors in breast cancer

Andrea Manni, Betty Badger, Carol Wright, Jerry Glenn, S. Rafeeq Ahmed, Laurence Demers

Research output: Contribution to journalArticle

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Abstract

Recent evidence indicates that autocrine/paracrine mechanisms may mediate the mitogenic effect of estradiol (E2) both in human and experimental breast cancer. However, the species-specificity of E2-regulated growth factors with regard to their biologic action has not been evaluated. To test this issue, we examined, in the soft agar clonogenic assay, the colony-stimulating activity in human breast cancers of conditioned media obtained from rat mammary carcinomas exposed to E2 (rat E2-CM). Of 22 primary human breast cancers plated in soft agar in the absence of serum, 18 (82%) successfully grew with a mean colony number of 62.4 ± 9.8 (S.E.M.) (range 14-193). Rat E2-CM significantly stimulated colony formation in 10 18 (56%) human breast cancers to 155 ± 11% (S.E.M.) of control. E2 administration (10-9 M) in these tumors had a virtually identical overall effect (154 ± 13% of control colony number). In the remaining eight tumors (44%), neither rat E2-CM nor E2 had, in general, a significant colony-stimulating effect. The growth-promoting action of rat E2-CM and E2 was not influenced by the hormone receptor status of the tumor. These results suggest that E2-regulated growth factors may not be species-specific, at least with regard to their colony-stimulating effects in soft agar.

Original languageEnglish (US)
Pages (from-to)1349-1354
Number of pages6
JournalEuropean Journal of Cancer and Clinical Oncology
Volume24
Issue number8
DOIs
StatePublished - Jan 1 1988

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Species Specificity
Estradiol
Intercellular Signaling Peptides and Proteins
Breast Neoplasms
Agar
Neoplasms
Conditioned Culture Medium
Human Activities
Hormones
Growth
Serum

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Manni, Andrea ; Badger, Betty ; Wright, Carol ; Glenn, Jerry ; Rafeeq Ahmed, S. ; Demers, Laurence. / Species-specificity of estradiol regulated growth factors in breast cancer. In: European Journal of Cancer and Clinical Oncology. 1988 ; Vol. 24, No. 8. pp. 1349-1354.
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Species-specificity of estradiol regulated growth factors in breast cancer. / Manni, Andrea; Badger, Betty; Wright, Carol; Glenn, Jerry; Rafeeq Ahmed, S.; Demers, Laurence.

In: European Journal of Cancer and Clinical Oncology, Vol. 24, No. 8, 01.01.1988, p. 1349-1354.

Research output: Contribution to journalArticle

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