Specific binding properties of 125I-apamin in various structures of the rat central nervous system

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Abstract

The properties of 125I-apamin binding with rat central nervous system slices were analysed in vitro using computerized densitometric autoradiography. Scatchard analysis performed for the data of binding experiments in rat brain and spinal cord demonstrates that apamin binds to a single class of non-interacting binding sites in all investigated structures. The dissociation constant values (K(D)) were similar in all investigated structures (31-38 pM). The maximal binding capacity (B(max)) was observed in the structures of limbic olfactory system (30 fmol/mg protein), the lowest in brain white matter (0.5 fmol/mg protein). It is concluded that the observed pattern of 125I-apamin binding might represent the topography of a class of Ca2+ dependent K+ channels in the rat central nervous system.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalActa Physiologica Polonica
Volume40
Issue number2
StatePublished - Dec 1 1989

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Apamin
Central Nervous System
Limbic System
Brain
Autoradiography
Spinal Cord
Proteins
Binding Sites

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

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abstract = "The properties of 125I-apamin binding with rat central nervous system slices were analysed in vitro using computerized densitometric autoradiography. Scatchard analysis performed for the data of binding experiments in rat brain and spinal cord demonstrates that apamin binds to a single class of non-interacting binding sites in all investigated structures. The dissociation constant values (K(D)) were similar in all investigated structures (31-38 pM). The maximal binding capacity (B(max)) was observed in the structures of limbic olfactory system (30 fmol/mg protein), the lowest in brain white matter (0.5 fmol/mg protein). It is concluded that the observed pattern of 125I-apamin binding might represent the topography of a class of Ca2+ dependent K+ channels in the rat central nervous system.",
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Specific binding properties of 125I-apamin in various structures of the rat central nervous system. / Janicki, P. K.

In: Acta Physiologica Polonica, Vol. 40, No. 2, 01.12.1989, p. 235-239.

Research output: Contribution to journalArticle

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