Specificity of developmental resistance in gypsy moth (Lymantria dispar) to two DNA-insect viruses

Kelli Hoover, Michael J. Grove

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Gypsy moth (Lymantria dispar) larvae displayed marked developmental resistance within an instar to L. dispar M nucleopolyhedrovirus (LdMNPV) regardless of the route of infection (oral or intrahemocoelic) in a previous study, indicating that in gypsy moth, this resistance has a systemic component. In this study, gypsy moth larvae challenged with the Amsacta moorei entomopoxvirus (AMEV) showed developmental resistance within the fourth instar to oral, but not intrahemocoelic, inoculation. In general, gypsy moth is considered refractory to oral challenge with AMEV, but in this study, 43% mortality occurred in newly molted fourth instars fed a dose of 5 × 106 large spheroids of AMEV; large spheroids were found to be more infectious than small spheroids when separated by a sucrose gradient. Developmental resistance within the fourth instar was reflected by a 2-fold reduction in mortality (18%-21%) with 5 × 106 large spheroids in larvae orally challenged at 24, 48 or 72 h post-molt. Fourth instars were highly sensitive to intrahemocoelic challenge with AMEV; 1PFU produced approximately 80% mortality regardless of age within the instar. These results indicate that in gypsy moth, systemic developmental resistance may be specific to LdMNPV, reflecting a co-evolutionary relationship between the baculovirus and its host.

Original languageEnglish (US)
Pages (from-to)493-500
Number of pages8
JournalVirologica Sinica
Volume24
Issue number5
DOIs
StatePublished - Sep 28 2009

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Insect Viruses
Entomopoxvirinae
DNA Viruses
Moths
Nucleopolyhedrovirus
Larva
Mortality
Baculoviridae
Sucrose
Infection

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology
  • Virology

Cite this

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title = "Specificity of developmental resistance in gypsy moth (Lymantria dispar) to two DNA-insect viruses",
abstract = "Gypsy moth (Lymantria dispar) larvae displayed marked developmental resistance within an instar to L. dispar M nucleopolyhedrovirus (LdMNPV) regardless of the route of infection (oral or intrahemocoelic) in a previous study, indicating that in gypsy moth, this resistance has a systemic component. In this study, gypsy moth larvae challenged with the Amsacta moorei entomopoxvirus (AMEV) showed developmental resistance within the fourth instar to oral, but not intrahemocoelic, inoculation. In general, gypsy moth is considered refractory to oral challenge with AMEV, but in this study, 43{\%} mortality occurred in newly molted fourth instars fed a dose of 5 × 106 large spheroids of AMEV; large spheroids were found to be more infectious than small spheroids when separated by a sucrose gradient. Developmental resistance within the fourth instar was reflected by a 2-fold reduction in mortality (18{\%}-21{\%}) with 5 × 106 large spheroids in larvae orally challenged at 24, 48 or 72 h post-molt. Fourth instars were highly sensitive to intrahemocoelic challenge with AMEV; 1PFU produced approximately 80{\%} mortality regardless of age within the instar. These results indicate that in gypsy moth, systemic developmental resistance may be specific to LdMNPV, reflecting a co-evolutionary relationship between the baculovirus and its host.",
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Specificity of developmental resistance in gypsy moth (Lymantria dispar) to two DNA-insect viruses. / Hoover, Kelli; Grove, Michael J.

In: Virologica Sinica, Vol. 24, No. 5, 28.09.2009, p. 493-500.

Research output: Contribution to journalArticle

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