Spectral hole burning of the primary electron donor state of Photosystem I

J. Kevin Gillie, Paul A. Lyle, Gerald J. Small, John H. Golbeck

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Abstract

Persistent photochemical hole burned profiles are reported for the primary electron donor state P700 of the reaction center of PS I. The hole profiles at 1.6 K for a wide range of burn wavelengths (λB) are broad (FWHM∼310 cm-1) and for the 45:1 enriched particles studied exhibit no sharp zero-phonon hole feature coincident with λB. The λB hole profiles are analyzed using the theory of Hayes et al. [J Phys Chem 1986, 90: 4928] for hole burning in the presence of arbitrarily strong linear electron-phonon coupling. A Huang-Rhys factor S in the range 4-6 and a corresponding mean phonon frequency in the range 35-50 cm-1 together with an inhomogeneous line broadening of∼100 cm-1 are found to provide good agreement with experiment. The zero-point level of P700* is predicted to lie at∼710 nm at 1.6K with an absorption maximum at∼702 nm. The hole spectra are discussed in the context of the hole spectra for the primary electron donor states of PS II and purple bacteria.

Original languageEnglish (US)
Pages (from-to)233-246
Number of pages14
JournalPhotosynthesis research
Volume22
Issue number3
DOIs
StatePublished - Dec 1 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

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