Spectral properties of single BODIPY dyes in polystyrene microspheres and in solutions

Bruce P. Wittmershaus, Jamie J. Skibicki, Jason B. McLafferty, Yu Zhong Zhang, Sharon Swan

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The absorption, fluorescence, fluorescence quantum yield, and photostability of five BODIPY dyes are characterized and compared as single dyes in two environments, in 40-nm polystyrene spheres and in solution. The absorption and fluorescence spectra of the dyes in spheres are similar in profile but shifted to lower energies compared to those in solution. All the dyes are highly fluorescent, with three having fluorescence quantum yields of 1.0. For three of the five dyes, the yields were the same in spheres as in solution (1.00, 1.00, and 0.73). The high concentration of these dyes in spheres does not quench their fluorescence. For two other dyes the yields dropped, from 1.00 to 0.55 in one case and 0.83 to 0.50 in another, comparing the dyes in solution versus in spheres. The photodegradation of the dyes decreases in spheres compared to in solution in all but one case. For one dye, it decreases as much as 800-fold. Dyes overlooked because of low fluorescence or stability in solution could become useful fluorescent materials in the microsphere environment.

Original languageEnglish (US)
Pages (from-to)119-128
Number of pages10
JournalJournal of Fluorescence
Volume11
Issue number2
DOIs
StatePublished - Jun 1 2001

Fingerprint

Polystyrenes
Microspheres
Coloring Agents
Fluorescence
energy
Quantum yield
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Photolysis
Photodegradation
Fluorescent Dyes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law

Cite this

Wittmershaus, Bruce P. ; Skibicki, Jamie J. ; McLafferty, Jason B. ; Zhang, Yu Zhong ; Swan, Sharon. / Spectral properties of single BODIPY dyes in polystyrene microspheres and in solutions. In: Journal of Fluorescence. 2001 ; Vol. 11, No. 2. pp. 119-128.
@article{34ece3f337fe47b2a2af9400c303a61b,
title = "Spectral properties of single BODIPY dyes in polystyrene microspheres and in solutions",
abstract = "The absorption, fluorescence, fluorescence quantum yield, and photostability of five BODIPY dyes are characterized and compared as single dyes in two environments, in 40-nm polystyrene spheres and in solution. The absorption and fluorescence spectra of the dyes in spheres are similar in profile but shifted to lower energies compared to those in solution. All the dyes are highly fluorescent, with three having fluorescence quantum yields of 1.0. For three of the five dyes, the yields were the same in spheres as in solution (1.00, 1.00, and 0.73). The high concentration of these dyes in spheres does not quench their fluorescence. For two other dyes the yields dropped, from 1.00 to 0.55 in one case and 0.83 to 0.50 in another, comparing the dyes in solution versus in spheres. The photodegradation of the dyes decreases in spheres compared to in solution in all but one case. For one dye, it decreases as much as 800-fold. Dyes overlooked because of low fluorescence or stability in solution could become useful fluorescent materials in the microsphere environment.",
author = "Wittmershaus, {Bruce P.} and Skibicki, {Jamie J.} and McLafferty, {Jason B.} and Zhang, {Yu Zhong} and Sharon Swan",
year = "2001",
month = "6",
day = "1",
doi = "10.1023/A:1016629518660",
language = "English (US)",
volume = "11",
pages = "119--128",
journal = "Journal of Fluorescence",
issn = "1053-0509",
number = "2",

}

Spectral properties of single BODIPY dyes in polystyrene microspheres and in solutions. / Wittmershaus, Bruce P.; Skibicki, Jamie J.; McLafferty, Jason B.; Zhang, Yu Zhong; Swan, Sharon.

In: Journal of Fluorescence, Vol. 11, No. 2, 01.06.2001, p. 119-128.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Spectral properties of single BODIPY dyes in polystyrene microspheres and in solutions

AU - Wittmershaus, Bruce P.

AU - Skibicki, Jamie J.

AU - McLafferty, Jason B.

AU - Zhang, Yu Zhong

AU - Swan, Sharon

PY - 2001/6/1

Y1 - 2001/6/1

N2 - The absorption, fluorescence, fluorescence quantum yield, and photostability of five BODIPY dyes are characterized and compared as single dyes in two environments, in 40-nm polystyrene spheres and in solution. The absorption and fluorescence spectra of the dyes in spheres are similar in profile but shifted to lower energies compared to those in solution. All the dyes are highly fluorescent, with three having fluorescence quantum yields of 1.0. For three of the five dyes, the yields were the same in spheres as in solution (1.00, 1.00, and 0.73). The high concentration of these dyes in spheres does not quench their fluorescence. For two other dyes the yields dropped, from 1.00 to 0.55 in one case and 0.83 to 0.50 in another, comparing the dyes in solution versus in spheres. The photodegradation of the dyes decreases in spheres compared to in solution in all but one case. For one dye, it decreases as much as 800-fold. Dyes overlooked because of low fluorescence or stability in solution could become useful fluorescent materials in the microsphere environment.

AB - The absorption, fluorescence, fluorescence quantum yield, and photostability of five BODIPY dyes are characterized and compared as single dyes in two environments, in 40-nm polystyrene spheres and in solution. The absorption and fluorescence spectra of the dyes in spheres are similar in profile but shifted to lower energies compared to those in solution. All the dyes are highly fluorescent, with three having fluorescence quantum yields of 1.0. For three of the five dyes, the yields were the same in spheres as in solution (1.00, 1.00, and 0.73). The high concentration of these dyes in spheres does not quench their fluorescence. For two other dyes the yields dropped, from 1.00 to 0.55 in one case and 0.83 to 0.50 in another, comparing the dyes in solution versus in spheres. The photodegradation of the dyes decreases in spheres compared to in solution in all but one case. For one dye, it decreases as much as 800-fold. Dyes overlooked because of low fluorescence or stability in solution could become useful fluorescent materials in the microsphere environment.

UR - http://www.scopus.com/inward/record.url?scp=0006289179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0006289179&partnerID=8YFLogxK

U2 - 10.1023/A:1016629518660

DO - 10.1023/A:1016629518660

M3 - Article

AN - SCOPUS:0006289179

VL - 11

SP - 119

EP - 128

JO - Journal of Fluorescence

JF - Journal of Fluorescence

SN - 1053-0509

IS - 2

ER -