Spectral resolution of the primary electron acceptor A0 in photosystem i

Adrien Chauvet, Naranbaatar Dashdorj, John H. Golbeck, T. Wade Johnson, Sergei Savikhin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The reduced state of the primary electron acceptor of Photosystem I, A 0, was resolved spectroscopically in its lowest energy Qy region for the first time without the addition of chemical reducing agents and without extensive data manipulation. To carry this out, we used the menB mutant of Synechocystis sp. PCC 6803 in which phylloquinone is replaced by plastoquinone-9 in the A1 sites of Photosystem I. The presence of plastoquinone-9 slows electron transfer from A0 to A1, leading to a long-lived A0- state. This allows its spectral signature to be readily detected in a time-resolved optical pump-probe experiment. The maximum bleaching (A0- - A0) was found to occur at 684 nm with a corresponding extinction coefficient of 43 mM-1 cm-1. The data show evidence for an electrochromic shift of an accessory chlorophyll pigment, suggesting that the latter Q y absorption band is centered around 682 nm.

Original languageEnglish (US)
Pages (from-to)3380-3386
Number of pages7
JournalJournal of Physical Chemistry B
Volume116
Issue number10
DOIs
StatePublished - Mar 15 2012

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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