Spectroscopic differentiation and quantification of microorganisms in apple juice

Chenxu Yu, Joseph Irudayaraj, Chitrita Debroy, Ze'ev Schmilovtich, Amos Mizrach

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A fast and easy-to-operate Fourier Transform Infrared (FTIR) spectrometry-based approach was developed for microbial differentiation and quantification in apple juice. Eight different microorganisms were evaluated: Enterobacter cloacae, Salmonella typhimurium, Enterobacter aerogenes, Salmonella choleraesuis, Serratia marcescens, Pseudomonas vulgaris, Vibrio cholerae, and Hafnia alvei. FTIR spectroscopy combined with chemometrics could differentiate the microorganisms studied at low concentration level of 103 colony-forming units (CFU)/ml in apple juice. The chemometric models developed to count microorganisms in apple juice were validated by an independent test set consisting of 18 samples and correlated against plate counts satisfactorily up to a detection limit of 103 CFU/mL.

Original languageEnglish (US)
Pages (from-to)268-272
Number of pages5
JournalJournal of Food Science
Volume69
Issue number7
StatePublished - Sep 2004

All Science Journal Classification (ASJC) codes

  • Food Science

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