Spectroscopic properties of chlorophyll a in the water-soluble peridinin-chlorophylla-protein complexes (PCP) from the symbiotic dinoflagellate Symbiodinium microadriaticum

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Abstract

The spectroscopic characteristics of chlorophyll (Chl) a in two water- soluble peridinin-chlorophyll a-protein complexes (PCP), isolated from the symbiotic dinoflagellate Symbiodinium microadriaticum, were analyzed in the 600 to 750 nm wavelength range. Gaussian deconvolution analyses of the absorption spectra of monomeric and dimeric PCP recorded at 160 K demonstrate that the Chl a Qy envelope of both complexes contains two spectral forms. Similar results were obtained from the Gaussian deconvolution of the fluorescence emission spectra taken at 119 K. Analyses of the calculated fluorescence emission of the different spectral forms at room temperature shows close agreement with the results obtained from Gaussian deconvolution analyses of the fluorescence emission spectra. The evidence indicates that the emission spectra of the intact complexes are the result of independent emission of each spectral form. Analyses of the contribution of each spectral form to the primary maximum of the emission spectra as a function of temperature is also supportive of the existence of two spectral forms of Chl a.

Original languageEnglish (US)
Pages (from-to)510-516
Number of pages7
JournalJournal of Plant Physiology
Volume149
Issue number5
DOIs
StatePublished - Jan 1 1996

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Dinoflagellida
Symbiodinium
Chlorophyll Binding Proteins
Fluorescence
chlorophyll
Water
Proteins
Temperature
proteins
water
fluorescence
Spectrum Analysis
chlorophyll a
peridinin
Miozoa
wavelengths
ambient temperature
temperature

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{3e43b22c56494fbba9def614fac0271c,
title = "Spectroscopic properties of chlorophyll a in the water-soluble peridinin-chlorophylla-protein complexes (PCP) from the symbiotic dinoflagellate Symbiodinium microadriaticum",
abstract = "The spectroscopic characteristics of chlorophyll (Chl) a in two water- soluble peridinin-chlorophyll a-protein complexes (PCP), isolated from the symbiotic dinoflagellate Symbiodinium microadriaticum, were analyzed in the 600 to 750 nm wavelength range. Gaussian deconvolution analyses of the absorption spectra of monomeric and dimeric PCP recorded at 160 K demonstrate that the Chl a Qy envelope of both complexes contains two spectral forms. Similar results were obtained from the Gaussian deconvolution of the fluorescence emission spectra taken at 119 K. Analyses of the calculated fluorescence emission of the different spectral forms at room temperature shows close agreement with the results obtained from Gaussian deconvolution analyses of the fluorescence emission spectra. The evidence indicates that the emission spectra of the intact complexes are the result of independent emission of each spectral form. Analyses of the contribution of each spectral form to the primary maximum of the emission spectra as a function of temperature is also supportive of the existence of two spectral forms of Chl a.",
author = "Roberto Iglesias-Prieto and Trench, {R. K.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/S0176-1617(96)80326-9",
language = "English (US)",
volume = "149",
pages = "510--516",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "5",

}

TY - JOUR

T1 - Spectroscopic properties of chlorophyll a in the water-soluble peridinin-chlorophylla-protein complexes (PCP) from the symbiotic dinoflagellate Symbiodinium microadriaticum

AU - Iglesias-Prieto, Roberto

AU - Trench, R. K.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The spectroscopic characteristics of chlorophyll (Chl) a in two water- soluble peridinin-chlorophyll a-protein complexes (PCP), isolated from the symbiotic dinoflagellate Symbiodinium microadriaticum, were analyzed in the 600 to 750 nm wavelength range. Gaussian deconvolution analyses of the absorption spectra of monomeric and dimeric PCP recorded at 160 K demonstrate that the Chl a Qy envelope of both complexes contains two spectral forms. Similar results were obtained from the Gaussian deconvolution of the fluorescence emission spectra taken at 119 K. Analyses of the calculated fluorescence emission of the different spectral forms at room temperature shows close agreement with the results obtained from Gaussian deconvolution analyses of the fluorescence emission spectra. The evidence indicates that the emission spectra of the intact complexes are the result of independent emission of each spectral form. Analyses of the contribution of each spectral form to the primary maximum of the emission spectra as a function of temperature is also supportive of the existence of two spectral forms of Chl a.

AB - The spectroscopic characteristics of chlorophyll (Chl) a in two water- soluble peridinin-chlorophyll a-protein complexes (PCP), isolated from the symbiotic dinoflagellate Symbiodinium microadriaticum, were analyzed in the 600 to 750 nm wavelength range. Gaussian deconvolution analyses of the absorption spectra of monomeric and dimeric PCP recorded at 160 K demonstrate that the Chl a Qy envelope of both complexes contains two spectral forms. Similar results were obtained from the Gaussian deconvolution of the fluorescence emission spectra taken at 119 K. Analyses of the calculated fluorescence emission of the different spectral forms at room temperature shows close agreement with the results obtained from Gaussian deconvolution analyses of the fluorescence emission spectra. The evidence indicates that the emission spectra of the intact complexes are the result of independent emission of each spectral form. Analyses of the contribution of each spectral form to the primary maximum of the emission spectra as a function of temperature is also supportive of the existence of two spectral forms of Chl a.

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U2 - 10.1016/S0176-1617(96)80326-9

DO - 10.1016/S0176-1617(96)80326-9

M3 - Article

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SP - 510

EP - 516

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 5

ER -