Spherical nanoparticle supported lipid bilayers: A tool for modeling protein interactions with curved membranes

Erin R. Tyndall, Fang Tian

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Mechanistic studies of protein-membrane interactions can be complicated by the limitations of the membrane model system chosen. Many of these limitations can be overcome by using a spherical silica nanoparticle to support the membrane. In this chapter, we present a detailed protocol for the construction of spherical nanoparticle supported lipid bilayers (SSLBs), with discussion of methods to improve production.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-109
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1688
ISSN (Print)1064-3745

Fingerprint

Lipid Bilayers
Nanoparticles
Membranes
Silicon Dioxide
Membrane Proteins
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Tyndall, E. R., & Tian, F. (2018). Spherical nanoparticle supported lipid bilayers: A tool for modeling protein interactions with curved membranes. In Methods in Molecular Biology (pp. 99-109). (Methods in Molecular Biology; Vol. 1688). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7386-6_5
Tyndall, Erin R. ; Tian, Fang. / Spherical nanoparticle supported lipid bilayers : A tool for modeling protein interactions with curved membranes. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 99-109 (Methods in Molecular Biology).
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Tyndall, ER & Tian, F 2018, Spherical nanoparticle supported lipid bilayers: A tool for modeling protein interactions with curved membranes. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1688, Humana Press Inc., pp. 99-109. https://doi.org/10.1007/978-1-4939-7386-6_5

Spherical nanoparticle supported lipid bilayers : A tool for modeling protein interactions with curved membranes. / Tyndall, Erin R.; Tian, Fang.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 99-109 (Methods in Molecular Biology; Vol. 1688).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Tyndall ER, Tian F. Spherical nanoparticle supported lipid bilayers: A tool for modeling protein interactions with curved membranes. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 99-109. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7386-6_5