Spin-echo based diagonal peak suppression in solid-state MAS NMR homonuclear chemical shift correlation spectra

Kaiyu Wang, Zhiyong Zhang, Xiaoyan Ding, Fang Tian, Yuqing Huang, Zhong Chen, Riqiang Fu

Research output: Contribution to journalArticle

Abstract

The feasibility of using the spin-echo based diagonal peak suppression method in solid-state MAS NMR homonuclear chemical shift correlation experiments is demonstrated. A complete phase cycling is designed in such a way that in the indirect dimension only the spin diffused signals are evolved, while all signals not involved in polarization transfer are refocused for cancellation. A data processing procedure is further introduced to reconstruct this acquired spectrum into a conventional two-dimensional homonuclear chemical shift correlation spectrum. A uniformly 13C, 15N labeled Fmoc-valine sample and the transmembrane domain of a human protein, LR11 (sorLA), in native Escherichia coli membranes have been used to illustrate the capability of the proposed method in comparison with standard 13C–13C chemical shift correlation experiments.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalJournal of Magnetic Resonance
Volume287
DOIs
StatePublished - Feb 1 2018

Fingerprint

Chemical shift
chemical equilibrium
echoes
Nuclear magnetic resonance
retarding
solid state
nuclear magnetic resonance
Valine
Escherichia
cancellation
Escherichia coli
Experiments
Polarization
membranes
proteins
Membranes
cycles
polarization
human SORL1 protein

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Cite this

Wang, Kaiyu ; Zhang, Zhiyong ; Ding, Xiaoyan ; Tian, Fang ; Huang, Yuqing ; Chen, Zhong ; Fu, Riqiang. / Spin-echo based diagonal peak suppression in solid-state MAS NMR homonuclear chemical shift correlation spectra. In: Journal of Magnetic Resonance. 2018 ; Vol. 287. pp. 91-98.
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Spin-echo based diagonal peak suppression in solid-state MAS NMR homonuclear chemical shift correlation spectra. / Wang, Kaiyu; Zhang, Zhiyong; Ding, Xiaoyan; Tian, Fang; Huang, Yuqing; Chen, Zhong; Fu, Riqiang.

In: Journal of Magnetic Resonance, Vol. 287, 01.02.2018, p. 91-98.

Research output: Contribution to journalArticle

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T1 - Spin-echo based diagonal peak suppression in solid-state MAS NMR homonuclear chemical shift correlation spectra

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AU - Ding, Xiaoyan

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AU - Chen, Zhong

AU - Fu, Riqiang

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AB - The feasibility of using the spin-echo based diagonal peak suppression method in solid-state MAS NMR homonuclear chemical shift correlation experiments is demonstrated. A complete phase cycling is designed in such a way that in the indirect dimension only the spin diffused signals are evolved, while all signals not involved in polarization transfer are refocused for cancellation. A data processing procedure is further introduced to reconstruct this acquired spectrum into a conventional two-dimensional homonuclear chemical shift correlation spectrum. A uniformly 13C, 15N labeled Fmoc-valine sample and the transmembrane domain of a human protein, LR11 (sorLA), in native Escherichia coli membranes have been used to illustrate the capability of the proposed method in comparison with standard 13C–13C chemical shift correlation experiments.

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