Spin-echo based diagonal peak suppression in solid-state MAS NMR homonuclear chemical shift correlation spectra

Kaiyu Wang, Zhiyong Zhang, Xiaoyan Ding, Fang Tian, Yuqing Huang, Zhong Chen, Riqiang Fu

Research output: Contribution to journalArticle

Abstract

The feasibility of using the spin-echo based diagonal peak suppression method in solid-state MAS NMR homonuclear chemical shift correlation experiments is demonstrated. A complete phase cycling is designed in such a way that in the indirect dimension only the spin diffused signals are evolved, while all signals not involved in polarization transfer are refocused for cancellation. A data processing procedure is further introduced to reconstruct this acquired spectrum into a conventional two-dimensional homonuclear chemical shift correlation spectrum. A uniformly 13C, 15N labeled Fmoc-valine sample and the transmembrane domain of a human protein, LR11 (sorLA), in native Escherichia coli membranes have been used to illustrate the capability of the proposed method in comparison with standard 13C–13C chemical shift correlation experiments.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalJournal of Magnetic Resonance
Volume287
DOIs
Publication statusPublished - Feb 2018

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Cite this