Spontaneous nucleosomal DNA fragmentation in murine leukemic L1210 cells

Cheng Jiang, Junxuan Lu, Gretchen Garcia, Henry J. Thompson

Research output: Contribution to journalArticle

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Abstract

Spontaneous nucleosomal DNA fragmentation (SNDF), characteristic of apoptotic cell death was observed in a murine leukemic cell line grown to high density. This effect was eliminated by permitting free diffusional exchange with a large amount of complete medium. Cells seeded at low density did not show SNDF in a conditioned medium that had been used to culture cells with severe SNDF. Cells seeded at high density in the same conditioned medium showed pronounced DNDF, but addition of 10% fetal bovine serum to such a medium significantly reduced SNDF. These observations indicate that neither physical contact among cells in high density culture per se nor secretion of a death factor by the high density culture could account for the induction of SNDF. The results support a model in which the gradual depletion of a serum factor(s) leads to the activation of a cell death program that is manifested as SNDF.

Original languageEnglish (US)
Pages (from-to)836-841
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume194
Issue number2
DOIs
StatePublished - Jul 30 1993

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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