Spontaneous nucleosomal DNA fragmentation in murine leukemic L1210 cells

Cheng Jiang, Junxuan Lu, Gretchen Garcia, Henry J. Thompson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Spontaneous nucleosomal DNA fragmentation (SNDF), characteristic of apoptotic cell death was observed in a murine leukemic cell line grown to high density. This effect was eliminated by permitting free diffusional exchange with a large amount of complete medium. Cells seeded at low density did not show SNDF in a conditioned medium that had been used to culture cells with severe SNDF. Cells seeded at high density in the same conditioned medium showed pronounced DNDF, but addition of 10% fetal bovine serum to such a medium significantly reduced SNDF. These observations indicate that neither physical contact among cells in high density culture per se nor secretion of a death factor by the high density culture could account for the induction of SNDF. The results support a model in which the gradual depletion of a serum factor(s) leads to the activation of a cell death program that is manifested as SNDF.

Original languageEnglish (US)
Pages (from-to)836-841
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume194
Issue number2
DOIs
StatePublished - Jul 30 1993

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DNA Fragmentation
DNA
Cell death
Conditioned Culture Medium
Cell Death
Serum
Cell culture
Cell Culture Techniques
Chemical activation
Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Spontaneous nucleosomal DNA fragmentation (SNDF), characteristic of apoptotic cell death was observed in a murine leukemic cell line grown to high density. This effect was eliminated by permitting free diffusional exchange with a large amount of complete medium. Cells seeded at low density did not show SNDF in a conditioned medium that had been used to culture cells with severe SNDF. Cells seeded at high density in the same conditioned medium showed pronounced DNDF, but addition of 10{\%} fetal bovine serum to such a medium significantly reduced SNDF. These observations indicate that neither physical contact among cells in high density culture per se nor secretion of a death factor by the high density culture could account for the induction of SNDF. The results support a model in which the gradual depletion of a serum factor(s) leads to the activation of a cell death program that is manifested as SNDF.",
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Spontaneous nucleosomal DNA fragmentation in murine leukemic L1210 cells. / Jiang, Cheng; Lu, Junxuan; Garcia, Gretchen; Thompson, Henry J.

In: Biochemical and Biophysical Research Communications, Vol. 194, No. 2, 30.07.1993, p. 836-841.

Research output: Contribution to journalArticle

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