Sprouting angiogenesis in engineered pseudo islets

Monika Hospodiuk, Madhuri Dey, Bugra Ayan, Donna Sosnoski, Kazim K. Moncal, Yang Wu, Ibrahim Tarik Ozbolat

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Despite the recent achievements in cell-based therapies for curing type-1 diabetes (T1D), capillarization in beta (β)-cell clusters is still a major roadblock as it is essential for long-Term viability and function of β-cells in vivo. In this research, we report sprouting angiogenesis in engineered pseudo islets (EPIs) made of mouse insulinoma βTC3 cells and rat heart microvascular endothelial cells (RHMVECs). Upon culturing in three-dimensional (3D) constructs under angiogenic conditions, EPIs sprouted extensive capillaries into the surrounding matrix. Ultra-morphological analysis through histological sections also revealed presence of capillarization within EPIs. EPIs cultured in 3D constructs maintained their viability and functionality over time while non-vascularized EPIs, without the presence of RHMVECs, could not retain their viability nor functionality. Here we demonstrate angiogenesis in engineered islets, where patient specific stem cell-derived human beta cells can be combined with microvascular endothelial cells in the near future for long-Term graft survival in T1D patients.

Original languageEnglish (US)
Article number035003
JournalBiofabrication
Volume10
Issue number3
DOIs
StatePublished - Mar 16 2018

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Endothelial cells
Endothelial Cells
Medical problems
Type 1 Diabetes Mellitus
Rats
Insulinoma
Graft Survival
Cell- and Tissue-Based Therapy
Stem cells
Grafts
Curing
Cell Survival
Stem Cells
Research

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Hospodiuk, M., Dey, M., Ayan, B., Sosnoski, D., Moncal, K. K., Wu, Y., & Ozbolat, I. T. (2018). Sprouting angiogenesis in engineered pseudo islets. Biofabrication, 10(3), [035003]. https://doi.org/10.1088/1758-5090/aab002
Hospodiuk, Monika ; Dey, Madhuri ; Ayan, Bugra ; Sosnoski, Donna ; Moncal, Kazim K. ; Wu, Yang ; Ozbolat, Ibrahim Tarik. / Sprouting angiogenesis in engineered pseudo islets. In: Biofabrication. 2018 ; Vol. 10, No. 3.
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Hospodiuk, M, Dey, M, Ayan, B, Sosnoski, D, Moncal, KK, Wu, Y & Ozbolat, IT 2018, 'Sprouting angiogenesis in engineered pseudo islets', Biofabrication, vol. 10, no. 3, 035003. https://doi.org/10.1088/1758-5090/aab002

Sprouting angiogenesis in engineered pseudo islets. / Hospodiuk, Monika; Dey, Madhuri; Ayan, Bugra; Sosnoski, Donna; Moncal, Kazim K.; Wu, Yang; Ozbolat, Ibrahim Tarik.

In: Biofabrication, Vol. 10, No. 3, 035003, 16.03.2018.

Research output: Contribution to journalArticle

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AU - Wu, Yang

AU - Ozbolat, Ibrahim Tarik

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Hospodiuk M, Dey M, Ayan B, Sosnoski D, Moncal KK, Wu Y et al. Sprouting angiogenesis in engineered pseudo islets. Biofabrication. 2018 Mar 16;10(3). 035003. https://doi.org/10.1088/1758-5090/aab002