Squalene Synthase Gene from Medicinal Herb Bacopa monniera: Molecular Characterization, Differential Expression, Comparative Modeling, and Docking Studies

Rishi K. Vishwakarma, Krunal Patel, Prashant Sonawane, Uma Kumari, Somesh Singh, Ruby, Shakeel Abbassi, Dinesh C. Agrawal, Hsin Sheng Tsay, Bashir M. Khan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The widespread pharmaceutically important triterpenoid saponins are synthesized via isoprenoid pathway. The formation of squalene is the key regulatory point in triterpene biosynthesis, catalyzed by squalene synthase (SQS). The present study deals with the detailed characterization of SQS by molecular, biochemical, and computational means from Bacopa monniera, an immensely important medicinal plant rich in triterpenoid saponin, bacosides. A full-length SQS gene was isolated from B. monniera, characterized as B. monniera squalene synthase (BmSQS) (1242 bp) encoding 414 amino acids. Deduced amino acid sequence of BmSQS showed highly conserved consensus aspartate-rich motifs (DXXXD) and catalytic site residues. Phylogenetic analysis showed that BmSQS belongs to dicot group having closest relationship with Salvia miltiorrhiza. Semiquantitative and real-time PCR studies showed that the BmSQS messenger RNA (mRNA) expression level was higher in vegetative parts (roots) as compared to floral parts. Methyl jasmonate induces the BmSQS mRNA expression in all tissues tested, while salicylic acid, cold, and salt induce much higher expression in roots. Homology modeling and docking simulations of BmSQS showed the pivotal roles of Asp77, Asp81, Asp213, Asp217, and Tyr168 in catalysis.

Original languageEnglish (US)
Pages (from-to)1675-1685
Number of pages11
JournalPlant Molecular Biology Reporter
Volume33
Issue number6
DOIs
StatePublished - Dec 1 2015

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Plant Science

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