2 Citations (Scopus)

Abstract

Background: Hernia repair is a common surgical procedure with polypropylene (PP) mesh being the standard material for correction because of its durability. However, complications such as seroma and pain are common, and repair failures still approach 15% secondary to poor tissue integration. In an effort to enhance mesh integration, we evaluated the applicability of a squid ring teeth (SRT) protein coating for soft-tissue repair in an abdominal wall defect model. SRT is a biologically derived high-strength protein with strong mechanical properties. We assessed tissue integration, strength, and biocompatibility of a SRT-coated PP mesh in a first-time pilot animal study. Methods: PP mesh was coated with SRT (SRT-PP) and tested for mechanical strength against uncoated PP mesh. Cell proliferation and adhesion studies were performed in vitro using a 3T3 cell line. Rats underwent either PP (n = 3) or SRTPP (n = 6) bridge mesh implantation in an anterior abdominal wall defect model. Repair was assessed clinically and radiographically, with integration evaluated by histology and mechanical testing at 60 days. Results: Cell proliferation was enhanced on SRT-PP mesh. This was corroborated in vivo by abdominal wall histology, dramatically diminished craniocaudal mesh contraction, improved strength testing, and higher tissue failure strain. There was no increase in seroma or visceral adhesion formation. No foreign body reactions were noted on liver histology. Conclusions: SRT applied as a coating appears to augment mesh-tissue integration and improve abdominal wall stability following bridged repair. Further studies in larger animals will determine its applicability for hernia repair in patients.

Original languageEnglish (US)
Article numbere1881
JournalPlastic and Reconstructive Surgery - Global Open
Volume6
Issue number8
DOIs
StatePublished - Jan 1 2018

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Decapodiformes
Polypropylenes
Abdominal Wall
Tooth
Seroma
Histology
Herniorrhaphy
Cell Proliferation
Foreign-Body Reaction
3T3 Cells
Cell Adhesion
Proteins
Pain
Cell Line
Liver

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Leberfinger, Ashley N. ; Hospodiuk, Monika ; Pena-Francesch, Abdon ; Ayan, Bugra ; Ozbolat, Veli ; Koduru, Srinivas V. ; Ozbolat, Ibrahim T. ; Demirel, Melik C. ; Ravnic, Dino J. / Squid ring teeth-coated mesh improves abdominal wall repair. In: Plastic and Reconstructive Surgery - Global Open. 2018 ; Vol. 6, No. 8.
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title = "Squid ring teeth-coated mesh improves abdominal wall repair",
abstract = "Background: Hernia repair is a common surgical procedure with polypropylene (PP) mesh being the standard material for correction because of its durability. However, complications such as seroma and pain are common, and repair failures still approach 15{\%} secondary to poor tissue integration. In an effort to enhance mesh integration, we evaluated the applicability of a squid ring teeth (SRT) protein coating for soft-tissue repair in an abdominal wall defect model. SRT is a biologically derived high-strength protein with strong mechanical properties. We assessed tissue integration, strength, and biocompatibility of a SRT-coated PP mesh in a first-time pilot animal study. Methods: PP mesh was coated with SRT (SRT-PP) and tested for mechanical strength against uncoated PP mesh. Cell proliferation and adhesion studies were performed in vitro using a 3T3 cell line. Rats underwent either PP (n = 3) or SRTPP (n = 6) bridge mesh implantation in an anterior abdominal wall defect model. Repair was assessed clinically and radiographically, with integration evaluated by histology and mechanical testing at 60 days. Results: Cell proliferation was enhanced on SRT-PP mesh. This was corroborated in vivo by abdominal wall histology, dramatically diminished craniocaudal mesh contraction, improved strength testing, and higher tissue failure strain. There was no increase in seroma or visceral adhesion formation. No foreign body reactions were noted on liver histology. Conclusions: SRT applied as a coating appears to augment mesh-tissue integration and improve abdominal wall stability following bridged repair. Further studies in larger animals will determine its applicability for hernia repair in patients.",
author = "Leberfinger, {Ashley N.} and Monika Hospodiuk and Abdon Pena-Francesch and Bugra Ayan and Veli Ozbolat and Koduru, {Srinivas V.} and Ozbolat, {Ibrahim T.} and Demirel, {Melik C.} and Ravnic, {Dino J.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1097/GOX.0000000000001881",
language = "English (US)",
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journal = "Plastic and Reconstructive Surgery - Global Open",
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Squid ring teeth-coated mesh improves abdominal wall repair. / Leberfinger, Ashley N.; Hospodiuk, Monika; Pena-Francesch, Abdon; Ayan, Bugra; Ozbolat, Veli; Koduru, Srinivas V.; Ozbolat, Ibrahim T.; Demirel, Melik C.; Ravnic, Dino J.

In: Plastic and Reconstructive Surgery - Global Open, Vol. 6, No. 8, e1881, 01.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Squid ring teeth-coated mesh improves abdominal wall repair

AU - Leberfinger, Ashley N.

AU - Hospodiuk, Monika

AU - Pena-Francesch, Abdon

AU - Ayan, Bugra

AU - Ozbolat, Veli

AU - Koduru, Srinivas V.

AU - Ozbolat, Ibrahim T.

AU - Demirel, Melik C.

AU - Ravnic, Dino J.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Hernia repair is a common surgical procedure with polypropylene (PP) mesh being the standard material for correction because of its durability. However, complications such as seroma and pain are common, and repair failures still approach 15% secondary to poor tissue integration. In an effort to enhance mesh integration, we evaluated the applicability of a squid ring teeth (SRT) protein coating for soft-tissue repair in an abdominal wall defect model. SRT is a biologically derived high-strength protein with strong mechanical properties. We assessed tissue integration, strength, and biocompatibility of a SRT-coated PP mesh in a first-time pilot animal study. Methods: PP mesh was coated with SRT (SRT-PP) and tested for mechanical strength against uncoated PP mesh. Cell proliferation and adhesion studies were performed in vitro using a 3T3 cell line. Rats underwent either PP (n = 3) or SRTPP (n = 6) bridge mesh implantation in an anterior abdominal wall defect model. Repair was assessed clinically and radiographically, with integration evaluated by histology and mechanical testing at 60 days. Results: Cell proliferation was enhanced on SRT-PP mesh. This was corroborated in vivo by abdominal wall histology, dramatically diminished craniocaudal mesh contraction, improved strength testing, and higher tissue failure strain. There was no increase in seroma or visceral adhesion formation. No foreign body reactions were noted on liver histology. Conclusions: SRT applied as a coating appears to augment mesh-tissue integration and improve abdominal wall stability following bridged repair. Further studies in larger animals will determine its applicability for hernia repair in patients.

AB - Background: Hernia repair is a common surgical procedure with polypropylene (PP) mesh being the standard material for correction because of its durability. However, complications such as seroma and pain are common, and repair failures still approach 15% secondary to poor tissue integration. In an effort to enhance mesh integration, we evaluated the applicability of a squid ring teeth (SRT) protein coating for soft-tissue repair in an abdominal wall defect model. SRT is a biologically derived high-strength protein with strong mechanical properties. We assessed tissue integration, strength, and biocompatibility of a SRT-coated PP mesh in a first-time pilot animal study. Methods: PP mesh was coated with SRT (SRT-PP) and tested for mechanical strength against uncoated PP mesh. Cell proliferation and adhesion studies were performed in vitro using a 3T3 cell line. Rats underwent either PP (n = 3) or SRTPP (n = 6) bridge mesh implantation in an anterior abdominal wall defect model. Repair was assessed clinically and radiographically, with integration evaluated by histology and mechanical testing at 60 days. Results: Cell proliferation was enhanced on SRT-PP mesh. This was corroborated in vivo by abdominal wall histology, dramatically diminished craniocaudal mesh contraction, improved strength testing, and higher tissue failure strain. There was no increase in seroma or visceral adhesion formation. No foreign body reactions were noted on liver histology. Conclusions: SRT applied as a coating appears to augment mesh-tissue integration and improve abdominal wall stability following bridged repair. Further studies in larger animals will determine its applicability for hernia repair in patients.

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DO - 10.1097/GOX.0000000000001881

M3 - Article

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JO - Plastic and Reconstructive Surgery - Global Open

JF - Plastic and Reconstructive Surgery - Global Open

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