sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis

Dorota A. Bulik, Ge Wei, Hidenao Toyoda, Akiko Kinoshita-Toyoda, W. Ross Waldrip, Jeffrey D. Esko, Phillips W. Robbins, Scott B. Selleck

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

sqv (squashed vulva) genes comprise a set of eight independent loci in Caenorhabditis elegans required zygotically for the invagination of vulval epithelial cells and maternally for normal oocyte formation and embryogenesis. Sequencing of sqv-3, sqv-7, and sqv-8 suggested a role for the encoded proteins in glycolipid or glycoprotein biosynthesis. Using a combination of in vitro analysis of SQV enzymatic activities, sqv+-mediated rescue of vertebrate cell lines, and biochemical characterization of sqv mutants, we show that sqv-3, -7, and -8 all affect the biosynthesis of glycosaminoglycans and therefore compromise the function of one specific class of glycoconjugates, proteoglycans. These findings establish the importance of proteoglycans and their associated glycosaminoglycans in epithelial morphogenesis and patterning during C. elegans development.

Original languageEnglish (US)
Pages (from-to)10838-10843
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number20
DOIs
StatePublished - Sep 26 2000

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Vulva
Caenorhabditis elegans
Glycosaminoglycans
Morphogenesis
Enzymes
Genes
Proteoglycans
Glycoconjugates
Glycolipids
Oocytes
Embryonic Development
Vertebrates
Glycoproteins
Epithelial Cells
Cell Line

All Science Journal Classification (ASJC) codes

  • General

Cite this

Bulik, Dorota A. ; Wei, Ge ; Toyoda, Hidenao ; Kinoshita-Toyoda, Akiko ; Waldrip, W. Ross ; Esko, Jeffrey D. ; Robbins, Phillips W. ; Selleck, Scott B. / sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 20. pp. 10838-10843.
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sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis. / Bulik, Dorota A.; Wei, Ge; Toyoda, Hidenao; Kinoshita-Toyoda, Akiko; Waldrip, W. Ross; Esko, Jeffrey D.; Robbins, Phillips W.; Selleck, Scott B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 20, 26.09.2000, p. 10838-10843.

Research output: Contribution to journalArticle

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AU - Bulik, Dorota A.

AU - Wei, Ge

AU - Toyoda, Hidenao

AU - Kinoshita-Toyoda, Akiko

AU - Waldrip, W. Ross

AU - Esko, Jeffrey D.

AU - Robbins, Phillips W.

AU - Selleck, Scott B.

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