Stability of long-chain and short-chain 3-hydroxyacyl-CoA dehydrogenase activity in postmortem liver

Daniel Hale, John E. Cornell, Michael J. Bennett

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Inherited enzyme defects in mitochondrial fatty acid oxidation (FAO) are associated with acute metabolic crisis and sudden death. Necropsy findings may be subtle, yielding no diagnosis and precluding genetic counseling. Preliminary identification of an FAO disorder requires the use of sophisticated tools (e.g., GC/MS) and specific body fluids, and the diagnosis rests on molecular analysis or enzyme assay. At present, confirmation of long-chain or short-chain 3-hydroxyacyl-CoA dehydrogenase deficiency relies on measurement of enzyme activity. Here, we report our examination of the effect of storage temperature (25, 4, -20, and -70 °C) and the postmortem interval on enzyme activities in rat and human liver. Enzyme activity decreases 50% in 30 h in samples stored at 25 °C, whereas 55 h at 4°C is required to reach this value; freezing minimizes this loss. Regardless of rate of degradation, however, the short-chain to long-chain activity ratio remains constant-which should make it possible to differentiate postmortem degradation from enzyme deficiency.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalClinical Chemistry
Volume43
Issue number2
StatePublished - Feb 20 1997

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3-Hydroxyacyl-CoA Dehydrogenase
Enzyme activity
Liver
Enzymes
Fatty Acids
Degradation
Oxidation
Body fluids
Freezing
Rats
Assays
Genetic Counseling
Enzyme Assays
Body Fluids
Defects
Sudden Death
Temperature

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Hale, Daniel ; Cornell, John E. ; Bennett, Michael J. / Stability of long-chain and short-chain 3-hydroxyacyl-CoA dehydrogenase activity in postmortem liver. In: Clinical Chemistry. 1997 ; Vol. 43, No. 2. pp. 273-278.
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Stability of long-chain and short-chain 3-hydroxyacyl-CoA dehydrogenase activity in postmortem liver. / Hale, Daniel; Cornell, John E.; Bennett, Michael J.

In: Clinical Chemistry, Vol. 43, No. 2, 20.02.1997, p. 273-278.

Research output: Contribution to journalArticle

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AB - Inherited enzyme defects in mitochondrial fatty acid oxidation (FAO) are associated with acute metabolic crisis and sudden death. Necropsy findings may be subtle, yielding no diagnosis and precluding genetic counseling. Preliminary identification of an FAO disorder requires the use of sophisticated tools (e.g., GC/MS) and specific body fluids, and the diagnosis rests on molecular analysis or enzyme assay. At present, confirmation of long-chain or short-chain 3-hydroxyacyl-CoA dehydrogenase deficiency relies on measurement of enzyme activity. Here, we report our examination of the effect of storage temperature (25, 4, -20, and -70 °C) and the postmortem interval on enzyme activities in rat and human liver. Enzyme activity decreases 50% in 30 h in samples stored at 25 °C, whereas 55 h at 4°C is required to reach this value; freezing minimizes this loss. Regardless of rate of degradation, however, the short-chain to long-chain activity ratio remains constant-which should make it possible to differentiate postmortem degradation from enzyme deficiency.

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