Stability of opioid growth factor ([Met5]-enkephalin) in solution using HPLC and photodiode array detection

W. Jeffrey Hurst, Patricia J. McLaughlin, Ian S. Zagon, Moshe Rogosnitzky

Research output: Contribution to journalArticlepeer-review

Abstract

A reversed phased HPLC method with photodiode array detection was used to monitor the stability of opioid growth factor (OGF; [Met5]- enkephalin) in sterile saline for 1, 2, and 8 weeks, and stored at -17°C, 4°C, and 23°C. Samples maintained at -17°C had 97% of the initial OGF concentration after 2 months, whereas those stored at 4°C had 94% of the original concentration. Preparations housed at 22°C had reductions of 6-12% by the 8th week. These data provide important information about storage of OGF for clinical applications.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalJournal of Liquid Chromatography and Related Technologies
Volume29
Issue number2
DOIs
StatePublished - Jan 1 2006

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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