Abstract
Calcium-calmodulin-dependent protein kinase II (CaMKII) promotes the maturation of retinotectal glutamatergic synapses in Xenopus. Whether CaMKII activity also controls morphological maturation of optic tectal neurons was tested using in vivo time-lapse imaging of single neurons over periods of up to 5 days. Dendritic arbor elaboration slows with maturation, in correlation with the onset of CaMKII expression. Elevating CaMKII activity in young neurons by viral expression of constitutively active CaMKII slowed dendritic growth to a rate comparable to that of mature neurons. CaMKII overexpression stabilized dendritic structure in more mature neurons, whereas CaMKII inhibition increased their dendritic growth. Thus, endogenous CaMKII activity limits dendritic growth and stabilizes dendrites, and it may act as an activity-dependent mediator of neuronal maturation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 222-226 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 279 |
| Issue number | 5348 |
| DOIs | |
| State | Published - Jan 9 1998 |
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Stabilization of dendritic arbor structure in vivo by CaMKII. / Wu, Gang Yi; Cline, Hollis T.
In: Science, Vol. 279, No. 5348, 09.01.1998, p. 222-226.Research output: Contribution to journal › Article
TY - JOUR
T1 - Stabilization of dendritic arbor structure in vivo by CaMKII
AU - Wu, Gang Yi
AU - Cline, Hollis T.
PY - 1998/1/9
Y1 - 1998/1/9
N2 - Calcium-calmodulin-dependent protein kinase II (CaMKII) promotes the maturation of retinotectal glutamatergic synapses in Xenopus. Whether CaMKII activity also controls morphological maturation of optic tectal neurons was tested using in vivo time-lapse imaging of single neurons over periods of up to 5 days. Dendritic arbor elaboration slows with maturation, in correlation with the onset of CaMKII expression. Elevating CaMKII activity in young neurons by viral expression of constitutively active CaMKII slowed dendritic growth to a rate comparable to that of mature neurons. CaMKII overexpression stabilized dendritic structure in more mature neurons, whereas CaMKII inhibition increased their dendritic growth. Thus, endogenous CaMKII activity limits dendritic growth and stabilizes dendrites, and it may act as an activity-dependent mediator of neuronal maturation.
AB - Calcium-calmodulin-dependent protein kinase II (CaMKII) promotes the maturation of retinotectal glutamatergic synapses in Xenopus. Whether CaMKII activity also controls morphological maturation of optic tectal neurons was tested using in vivo time-lapse imaging of single neurons over periods of up to 5 days. Dendritic arbor elaboration slows with maturation, in correlation with the onset of CaMKII expression. Elevating CaMKII activity in young neurons by viral expression of constitutively active CaMKII slowed dendritic growth to a rate comparable to that of mature neurons. CaMKII overexpression stabilized dendritic structure in more mature neurons, whereas CaMKII inhibition increased their dendritic growth. Thus, endogenous CaMKII activity limits dendritic growth and stabilizes dendrites, and it may act as an activity-dependent mediator of neuronal maturation.
UR - http://www.scopus.com/inward/record.url?scp=0032498213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032498213&partnerID=8YFLogxK
U2 - 10.1126/science.279.5348.222
DO - 10.1126/science.279.5348.222
M3 - Article
C2 - 9422694
AN - SCOPUS:0032498213
VL - 279
SP - 222
EP - 226
JO - Science
JF - Science
SN - 0036-8075
IS - 5348
ER -