Standing surface acoustic wave based cell coculture

Sixing Li, Feng Guo, Yuchao Chen, Xiaoyun Ding, Peng Li, Lin Wang, Craig Eugene Cameron, Tony Jun Huang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

Original languageEnglish (US)
Pages (from-to)9853-9859
Number of pages7
JournalAnalytical Chemistry
Volume86
Issue number19
DOIs
StatePublished - Oct 7 2014

Fingerprint

Surface waves
Acoustic waves
Endothelial cells
Cells
Controllability
Biocompatibility
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Li, S., Guo, F., Chen, Y., Ding, X., Li, P., Wang, L., ... Huang, T. J. (2014). Standing surface acoustic wave based cell coculture. Analytical Chemistry, 86(19), 9853-9859. https://doi.org/10.1021/ac502453z
Li, Sixing ; Guo, Feng ; Chen, Yuchao ; Ding, Xiaoyun ; Li, Peng ; Wang, Lin ; Cameron, Craig Eugene ; Huang, Tony Jun. / Standing surface acoustic wave based cell coculture. In: Analytical Chemistry. 2014 ; Vol. 86, No. 19. pp. 9853-9859.
@article{9da5f7e02865453cbad8411ec7250619,
title = "Standing surface acoustic wave based cell coculture",
abstract = "Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.",
author = "Sixing Li and Feng Guo and Yuchao Chen and Xiaoyun Ding and Peng Li and Lin Wang and Cameron, {Craig Eugene} and Huang, {Tony Jun}",
year = "2014",
month = "10",
day = "7",
doi = "10.1021/ac502453z",
language = "English (US)",
volume = "86",
pages = "9853--9859",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "19",

}

Li, S, Guo, F, Chen, Y, Ding, X, Li, P, Wang, L, Cameron, CE & Huang, TJ 2014, 'Standing surface acoustic wave based cell coculture', Analytical Chemistry, vol. 86, no. 19, pp. 9853-9859. https://doi.org/10.1021/ac502453z

Standing surface acoustic wave based cell coculture. / Li, Sixing; Guo, Feng; Chen, Yuchao; Ding, Xiaoyun; Li, Peng; Wang, Lin; Cameron, Craig Eugene; Huang, Tony Jun.

In: Analytical Chemistry, Vol. 86, No. 19, 07.10.2014, p. 9853-9859.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Standing surface acoustic wave based cell coculture

AU - Li, Sixing

AU - Guo, Feng

AU - Chen, Yuchao

AU - Ding, Xiaoyun

AU - Li, Peng

AU - Wang, Lin

AU - Cameron, Craig Eugene

AU - Huang, Tony Jun

PY - 2014/10/7

Y1 - 2014/10/7

N2 - Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

AB - Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

UR - http://www.scopus.com/inward/record.url?scp=84907937855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907937855&partnerID=8YFLogxK

U2 - 10.1021/ac502453z

DO - 10.1021/ac502453z

M3 - Article

VL - 86

SP - 9853

EP - 9859

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 19

ER -

Li S, Guo F, Chen Y, Ding X, Li P, Wang L et al. Standing surface acoustic wave based cell coculture. Analytical Chemistry. 2014 Oct 7;86(19):9853-9859. https://doi.org/10.1021/ac502453z