Standing surface acoustic wave based cell coculture

Sixing Li, Feng Guo, Yuchao Chen, Xiaoyun Ding, Peng Li, Lin Wang, Craig E. Cameron, Tony Jun Huang

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

Original languageEnglish (US)
Pages (from-to)9853-9859
Number of pages7
JournalAnalytical chemistry
Volume86
Issue number19
DOIs
StatePublished - Oct 7 2014

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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    Li, S., Guo, F., Chen, Y., Ding, X., Li, P., Wang, L., Cameron, C. E., & Huang, T. J. (2014). Standing surface acoustic wave based cell coculture. Analytical chemistry, 86(19), 9853-9859. https://doi.org/10.1021/ac502453z