Standing surface acoustic wave (SSAW)-based cell washing

Sixing Li, Xiaoyun Ding, Zhangming Mao, Yuchao Chen, Nitesh Nama, Feng Guo, Peng Li, Lin Wang, Craig E. Cameron, Tony Jun Huang

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalLab on a Chip
Volume15
Issue number1
DOIs
StatePublished - Jan 7 2015

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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