Staphylococcal enterotoxin D production by Staphylococcus aureus FRI 100

N. M. Kauffman, R. F. Roberts

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Staphylococcus aureus FRI 100 is commonly used as a control strain for staphylococcal enterotoxin A (SEA) assays. When FRI 100 was used in PCR-based enterotoxin detection methods, the strain gave a positive result for both SEA and staphylococcal enterotoxin D (SED). Production of SED was confirmed by testing concentrated and unconcentrated culture supernatants with the TECRA staphylococcal enterotoxin visual immunoassay. SED was detected after 24 h of growth in Trypticase soy broth. Primers were created to amplify the entire sed gene by PCR for subsequent sequencing. The sequenced gene showed high similarity to a previously sequenced sed gene. The SED-like gene in FRI 100 exhibited four point mutations and two deletions. Changes in the FRI 100 open reading frame altered the primary structure of the SED-like protein, allowing for coding of only the first 150 amino acids followed by a stop codon. Because the SED active site is at the proximal end, where there was no change in DNA sequence, we conclude FRI 100 produces a variant form of SED. It is necessary to note that, when using FRI 100 as an SEA control strain, it does produce a variant of the SED protein, which exhibits immunological activity, and the sed-like gene is detected by commonly used PCR primers. This phenomenon may be an important general consideration when using PCR to characterize strains of toxin-producing S. aureus. S. aureus enterotoxin-positive PCR results should be confirmed by immunological techniques.

Original languageEnglish (US)
Pages (from-to)1448-1451
Number of pages4
JournalJournal of Food Protection
Volume69
Issue number6
DOIs
StatePublished - Jun 2006

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Fingerprint

Dive into the research topics of 'Staphylococcal enterotoxin D production by Staphylococcus aureus FRI 100'. Together they form a unique fingerprint.

Cite this