Starvation results in decreased initiation factor activity in rat skeletal muscle

D. Eugene Rannels, Anthony Pegg, Stephen R. Rannels

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Activity of a factor forming a nitrocellulose filter-bound complex with [35S]met-tRNAmet f and GTP was estimated in the post-ribosomal supernatant of psoas and heart muscle. Complex formation required initiator tRNA and GTP or GMP-P(CH2)P; it was inhibited by Mg2+, GDP, aurintricarboxylic acid, spermine and spermidine. Starvation reduced complex-forming activity of supernatants from psoas but not heart muscle. Reduced activity was not due to increased dilution or deacylation of met-tRNAmet f or to increased hydrolysis of GTP by extracts from starved animals.

Original languageEnglish (US)
Pages (from-to)1481-1489
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume72
Issue number4
DOIs
StatePublished - Oct 18 1976

Fingerprint

Peptide Initiation Factors
Starvation
Guanosine Triphosphate
Muscle
Rats
Skeletal Muscle
Myocardium
Aurintricarboxylic Acid
RNA, Transfer, Met
Psoas Muscles
Collodion
Spermidine
Spermine
Dilution
Hydrolysis
Animals

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Starvation results in decreased initiation factor activity in rat skeletal muscle. / Eugene Rannels, D.; Pegg, Anthony; Rannels, Stephen R.

In: Biochemical and Biophysical Research Communications, Vol. 72, No. 4, 18.10.1976, p. 1481-1489.

Research output: Contribution to journalArticle

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