Static time-of-flight secondary ion mass spectrometry imaging of freeze-fractured, frozen-hydrated biological membranes

Michaeleen L. Pacholski, Donald M. Cannon, Andrew G. Ewing, Nicholas Winograd

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

The study of cell membrane lipid and steroid composition and distribution is important for the understanding of membrane dynamics and function. Here we present efforts to chemically image phospholipid distributions on a submicron scale on freeze-fractured and frozen-hydrated liposomes and red blood cells using time-of-flight secondary ion mass spectrometry. Sample preparation by freeze fracturing of membranes is described. Fragments representative of phospholipid headgroups are found to be localized on both liposomes and red blood cells. In addition, the cholesterol molecular ion [M+H] is localized on liposome surfaces.

Original languageEnglish (US)
Pages (from-to)1232-1235
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume12
Issue number18
DOIs
StatePublished - 1998

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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