Stereochemistry of the decarboxylation of l-ornithine with ornithine decarboxylase from mouse kidney

Gary R. Orr, Steven J. Gould, Anthony E. Pegg, James E. Seely, James K. Coward

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Using highly purified ornithine decarboxylase isolated from androgen-treated mice, [1R-2H]putrescine was generated by the decarboxylation of l-ornithine in D2O, and [1S-2H]putrescine was generated from [2-2H]ornithine by carrying out the decarboxylation in H2O. Chirality of the putrescines was then determined from the 200-MHz 1H NMR spectra of their bis-camphanamides in the presence of Eu(fod)3. These results demonstrated that decarboxylation had taken place with retention of configuration.

Original languageEnglish (US)
Pages (from-to)252-258
Number of pages7
JournalBioorganic Chemistry
Issue number3
StatePublished - Sep 1984

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

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