Steroid metabolism by the lung: Conversion of dihydrotestosterone to 5α-androstan-3α, 17β-diol by rat lung tissue in vitro

Lidia Wei Liu Kao, M. Jane Crosswell, Judith Weisz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The potential of lung tissue of adult male rats to metabolize dihydrotestosterone (DHT) in vitro was examined. Within 3 min, a homogenate of 100 mg lung tissue, cleared of blood by perfusion before homogenization, metabolized 90% of the [3H]DHT substrate. Approximately 80% of the DHT was converted to 5α-androstan-3α, 17β-diol. The amount of 5α-androstan- 3α, 17β-diol formed during a 5-min incubation increased linearly, with substrate concentrations ranging from 3.3 × 10-8 to 3 × 10-6 M. Thus, the capacity of rat lung tissue to metabolize DHT in vitro and the rate of 3α-reduction of DHT are sufficiently great to consider the possibility that lung may be responsible for the rapid clearance of DHT from the circulation in this species.

Original languageEnglish (US)
Pages (from-to)664-666
Number of pages3
JournalEndocrinology
Volume104
Issue number3
DOIs
StatePublished - Jan 1 1979

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Dihydrotestosterone
Steroids
Lung
Lung Volume Measurements
In Vitro Techniques
Perfusion

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

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abstract = "The potential of lung tissue of adult male rats to metabolize dihydrotestosterone (DHT) in vitro was examined. Within 3 min, a homogenate of 100 mg lung tissue, cleared of blood by perfusion before homogenization, metabolized 90{\%} of the [3H]DHT substrate. Approximately 80{\%} of the DHT was converted to 5α-androstan-3α, 17β-diol. The amount of 5α-androstan- 3α, 17β-diol formed during a 5-min incubation increased linearly, with substrate concentrations ranging from 3.3 × 10-8 to 3 × 10-6 M. Thus, the capacity of rat lung tissue to metabolize DHT in vitro and the rate of 3α-reduction of DHT are sufficiently great to consider the possibility that lung may be responsible for the rapid clearance of DHT from the circulation in this species.",
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Steroid metabolism by the lung : Conversion of dihydrotestosterone to 5α-androstan-3α, 17β-diol by rat lung tissue in vitro. / Kao, Lidia Wei Liu; Crosswell, M. Jane; Weisz, Judith.

In: Endocrinology, Vol. 104, No. 3, 01.01.1979, p. 664-666.

Research output: Contribution to journalArticle

TY - JOUR

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T2 - Conversion of dihydrotestosterone to 5α-androstan-3α, 17β-diol by rat lung tissue in vitro

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