Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

Ruoyu He, Zhiping Liu, Yongkui Xu, Wei Huang, Hong Ma, Minbiao Ji

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

Original languageEnglish (US)
Pages (from-to)659-662
Number of pages4
JournalOptics Letters
Volume42
Issue number4
DOIs
StatePublished - Feb 15 2017

Fingerprint

delay lines
Raman spectra
microscopy
scanning
spectroscopy
tuning
pollen
rice
chemical analysis
artifacts
low concentrations
acquisition
high speed
mirrors
color
cells

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

He, Ruoyu ; Liu, Zhiping ; Xu, Yongkui ; Huang, Wei ; Ma, Hong ; Ji, Minbiao. / Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line. In: Optics Letters. 2017 ; Vol. 42, No. 4. pp. 659-662.
@article{762a58fd68cd439ca36c20c81f5d3763,
title = "Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line",
abstract = "Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.",
author = "Ruoyu He and Zhiping Liu and Yongkui Xu and Wei Huang and Hong Ma and Minbiao Ji",
year = "2017",
month = "2",
day = "15",
doi = "10.1364/OL.42.000659",
language = "English (US)",
volume = "42",
pages = "659--662",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "4",

}

Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line. / He, Ruoyu; Liu, Zhiping; Xu, Yongkui; Huang, Wei; Ma, Hong; Ji, Minbiao.

In: Optics Letters, Vol. 42, No. 4, 15.02.2017, p. 659-662.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

AU - He, Ruoyu

AU - Liu, Zhiping

AU - Xu, Yongkui

AU - Huang, Wei

AU - Ma, Hong

AU - Ji, Minbiao

PY - 2017/2/15

Y1 - 2017/2/15

N2 - Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

AB - Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

UR - http://www.scopus.com/inward/record.url?scp=85013427835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013427835&partnerID=8YFLogxK

U2 - 10.1364/OL.42.000659

DO - 10.1364/OL.42.000659

M3 - Article

C2 - 28198892

AN - SCOPUS:85013427835

VL - 42

SP - 659

EP - 662

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 4

ER -