Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

Ruoyu He, Zhiping Liu, Yongkui Xu, Wei Huang, Hong Ma, Minbiao Ji

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

Original languageEnglish (US)
Pages (from-to)659-662
Number of pages4
JournalOptics Letters
Volume42
Issue number4
DOIs
StatePublished - Feb 15 2017

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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