Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

Ruoyu He; Zhiping Liu; Yongkui Xu; Wei Huang; Hong Ma; Minbiao Ji

doi:10.1364/OL.42.000659

Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

Ruoyu He, Zhiping Liu, Yongkui Xu, Wei Huang, Hong Ma, Minbiao Ji

Huck Institutes of the Life Sciences

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

Original language	English (US)
Pages (from-to)	659-662
Number of pages	4
Journal	Optics Letters
Volume	42
Issue number	4
DOIs	https://doi.org/10.1364/OL.42.000659
State	Published - Feb 15 2017

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OL.42.000659

Cite this

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title = "Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line",

abstract = "Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.",

author = "Ruoyu He and Zhiping Liu and Yongkui Xu and Wei Huang and Hong Ma and Minbiao Ji",

note = "Funding Information: National Natural Science Foundation of China (NSFC) (81671725); Shanghai Rising Star Program (15QA1400500); Shanghai Action Plan for Scientific and Technological Innovation Program (16441909200); National Key Research and Development Program of China (2016YFA0301000, 2016YFC0102100). Publisher Copyright: {\textcopyright} 2017 Optical Society of America.",

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doi = "10.1364/OL.42.000659",

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T1 - Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

AU - He, Ruoyu

AU - Liu, Zhiping

AU - Xu, Yongkui

AU - Huang, Wei

AU - Ma, Hong

AU - Ji, Minbiao

N1 - Funding Information: National Natural Science Foundation of China (NSFC) (81671725); Shanghai Rising Star Program (15QA1400500); Shanghai Action Plan for Scientific and Technological Innovation Program (16441909200); National Key Research and Development Program of China (2016YFA0301000, 2016YFC0102100). Publisher Copyright: © 2017 Optical Society of America.

PY - 2017/2/15

Y1 - 2017/2/15

N2 - Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

AB - Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest.

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Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

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