Store-operated Ca2+ entry: Evidence for a secretion-like coupling model

Randen L. Patterson, Damian B. Van Rossum, Donald L. Gill

Research output: Contribution to journalArticle

373 Citations (Scopus)

Abstract

The elusive coupling between endoplasmic reticulum (ER) Ca2+ stores and plasma membrane (PM) "store-operated" Ca2+ entry channels was probed through a novel combination of cytoskeletal modifications. Whereas coupling was unaffected by disassembly of the actin cytoskeleton, in situ redistribution of F-actin into a tight cortical layer subjacent to the PM displaced cortical ER and prevented coupling between ER and PM Ca2+ entry channels, while not affecting inositol 1,4,5-trisphosphate-mediated store release. Importantly, disassembly of the induced cortical actin layer allowed ER to regain access to the PM and reestablish coupling of Ca2+ entry channels to Ca2+ store depletion. Coupling is concluded to be mediated by a physical "secretion-like" mechanism involving close but reversible interactions between the ER and the PM.

Original languageEnglish (US)
Pages (from-to)487-499
Number of pages13
JournalCell
Volume98
Issue number4
DOIs
StatePublished - Aug 20 1999

Fingerprint

Cell membranes
Endoplasmic Reticulum
Cell Membrane
Actins
Regain
Inositol 1,4,5-Trisphosphate
Actin Cytoskeleton

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Store-operated Ca2+ entry: Evidence for a secretion-like coupling model",
abstract = "The elusive coupling between endoplasmic reticulum (ER) Ca2+ stores and plasma membrane (PM) {"}store-operated{"} Ca2+ entry channels was probed through a novel combination of cytoskeletal modifications. Whereas coupling was unaffected by disassembly of the actin cytoskeleton, in situ redistribution of F-actin into a tight cortical layer subjacent to the PM displaced cortical ER and prevented coupling between ER and PM Ca2+ entry channels, while not affecting inositol 1,4,5-trisphosphate-mediated store release. Importantly, disassembly of the induced cortical actin layer allowed ER to regain access to the PM and reestablish coupling of Ca2+ entry channels to Ca2+ store depletion. Coupling is concluded to be mediated by a physical {"}secretion-like{"} mechanism involving close but reversible interactions between the ER and the PM.",
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Store-operated Ca2+ entry : Evidence for a secretion-like coupling model. / Patterson, Randen L.; Van Rossum, Damian B.; Gill, Donald L.

In: Cell, Vol. 98, No. 4, 20.08.1999, p. 487-499.

Research output: Contribution to journalArticle

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AU - Patterson, Randen L.

AU - Van Rossum, Damian B.

AU - Gill, Donald L.

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