Eight strains of Aspergillus screened for glucose production from industrial potato waste which will be used for ethanol fermentation. Aspergillus niger NRRL 330 was found the best strain among tested ones, with 121 U/ml amylase activity and 20.45 g/L glucose conversion. After strain selection, medium is screened for nitrogen sources and inorganic salts using Plackett-Burman design. Malt extract, FeSO4.7H20, and CaCl2.2H20 have positively significant effect while yeast extract and CaCO3 showed a negative effect on amylase production by A. niger on a waste potato mash medium under studied condition. Yeast extract, malt extract, MgSO4.7H20, and FeSO4.7H20 have positively significant effect while (NH4)2. SO4, KH2PO4, and CaCl2.2H20 showed a negative effect on ethanol production under studied conditions. Finally, a medium consisted of waste potato mash, malt extract, yeast extract, MgSO4.7H20, CaCl2.2H20 and FeSO4.7H20 would promote the microbial growth as well as higher yields of ethanol and amylase activity for simultaneous saccharification and fermentation of waste potato mash.